BACKGROUND: Ceftiofur is an effective antibiotic against respiratory infections in livestock. However, ceftiofur concentration that is found in lungs after intravenous injection is not effective. Fortunately, ceftiofur-loaded gelatin microsphere (Cef-MS) enjoys advantages of lung-targeting and can achieve an effective concentration. However, no study has been reported on this modality of drug delivery. OBJECTIVE: We investigated the properties of this delivery modality--lung targeting ceftiofur-Cef-MSs. METHODS: We prepared Cef-MS and investigated drug loading, stability and release characteristics in vitro and studied tissue distribution patterns and potential lung injury in mice. RESULTS: Results showed that the average size and span value are 21.26 μm and 1.07, respectively. Drug loading and loading efficiency were 15.31 and 76.55%, respectively. Cef-MSs were stable in light, heat and humidity, except that agglutinative phenomenon was observed in 90% humidity after 10 days. Cef-MS presented a slower in vitro release pattern compared to ceftiofur. Cef-MS mainly concentrates in lungs after intravenous administration. Furthermore, histopathological studies showed that Cef-MS only induces mild and reversible lung injury and is biologically safe. CONCLUSION: Cef-MS is a promising alternative form with high lung-targeting properties for the treatment of respiratory infections.
BACKGROUND:Ceftiofur is an effective antibiotic against respiratory infections in livestock. However, ceftiofur concentration that is found in lungs after intravenous injection is not effective. Fortunately, ceftiofur-loaded gelatin microsphere (Cef-MS) enjoys advantages of lung-targeting and can achieve an effective concentration. However, no study has been reported on this modality of drug delivery. OBJECTIVE: We investigated the properties of this delivery modality--lung targeting ceftiofur-Cef-MSs. METHODS: We prepared Cef-MS and investigated drug loading, stability and release characteristics in vitro and studied tissue distribution patterns and potential lung injury in mice. RESULTS: Results showed that the average size and span value are 21.26 μm and 1.07, respectively. Drug loading and loading efficiency were 15.31 and 76.55%, respectively. Cef-MSs were stable in light, heat and humidity, except that agglutinative phenomenon was observed in 90% humidity after 10 days. Cef-MS presented a slower in vitro release pattern compared to ceftiofur. Cef-MS mainly concentrates in lungs after intravenous administration. Furthermore, histopathological studies showed that Cef-MS only induces mild and reversible lung injury and is biologically safe. CONCLUSION: Cef-MS is a promising alternative form with high lung-targeting properties for the treatment of respiratory infections.
Authors: Itishri Sahu; A K M Ashiqul Haque; Brian Weidensee; Petra Weinmann; Michael S D Kormann Journal: Mol Ther Date: 2019-03-06 Impact factor: 11.454
Authors: Susanne R Youngren; Rakesh K Tekade; Brianne Gustilo; Peter R Hoffmann; Mahavir B Chougule Journal: Biomed Res Int Date: 2013-09-26 Impact factor: 3.411