Literature DB >> 21632902

Use of modified PCR ribotyping for direct detection of Clostridium difficile ribotypes in stool samples.

Sandra Janezic1, Iztok Strumbelj, Maja Rupnik.   

Abstract

PCR ribotyping was modified to allow direct detection of Clostridium difficile from stool samples. Direct PCR ribotyping was possible in 86 out of 99 C. difficile-positive stool samples, and in 84 cases (84.8%), the ribotype determined directly from the stool sample was identical to the ribotype of the strain isolated from the same stool sample.

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Year:  2011        PMID: 21632902      PMCID: PMC3147761          DOI: 10.1128/JCM.01013-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  5 in total

1.  Molecular typing methods for Clostridium difficile: pulsed-field gel electrophoresis and PCR ribotyping.

Authors:  Sandra Janezic; Maja Rupnik
Journal:  Methods Mol Biol       Date:  2010

2.  PCR targeted to the 16S-23S rRNA gene intergenic spacer region of Clostridium difficile and construction of a library consisting of 116 different PCR ribotypes.

Authors:  S L Stubbs; J S Brazier; G L O'Neill; B I Duerden
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

3.  Development of a new PCR-ribotyping method for Clostridium difficile based on ribosomal RNA gene sequencing.

Authors:  P Bidet; F Barbut; V Lalande; B Burghoffer; J C Petit
Journal:  FEMS Microbiol Lett       Date:  1999-06-15       Impact factor: 2.742

4.  Coexistence of multiple PCR-ribotype strains of Clostridium difficile in faecal samples limits epidemiological studies.

Authors:  Renate J van den Berg; Hadi Aa Ameen; Takahiro Furusawa; Eric Cj Claas; Eric R van der Vorm; Ed J Kuijper
Journal:  J Med Microbiol       Date:  2005-02       Impact factor: 2.472

5.  Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.

Authors:  A Indra; S Huhulescu; M Schneeweis; P Hasenberger; S Kernbichler; A Fiedler; G Wewalka; F Allerberger; E J Kuijper
Journal:  J Med Microbiol       Date:  2008-11       Impact factor: 2.472

  5 in total
  5 in total

1.  A Clostridium difficile Cell Wall Glycopolymer Locus Influences Bacterial Shape, Polysaccharide Production and Virulence.

Authors:  Michele Chu; Michael J G Mallozzi; Bryan P Roxas; Lisa Bertolo; Mario A Monteiro; Al Agellon; V K Viswanathan; Gayatri Vedantam
Journal:  PLoS Pathog       Date:  2016-10-14       Impact factor: 6.823

2.  A global to local genomics analysis of Clostridioides difficile ST1/RT027 identifies cryptic transmission events in a northern Arizona healthcare network.

Authors:  Charles H D Williamson; Nathan E Stone; Amalee E Nunnally; Heidie M Hornstra; David M Wagner; Chandler C Roe; Adam J Vazquez; Nivedita Nandurkar; Jacob Vinocur; Joel Terriquez; John Gillece; Jason Travis; Darrin Lemmer; Paul Keim; Jason W Sahl
Journal:  Microb Genom       Date:  2019-05-20

3.  Distribution of Clostridium Difficile Ribotypes in Macedonian Patients and their Antimicrobial Susceptibility.

Authors:  Kiril Mihajlov; Aneta Andreska; Nadica Ristovska; Tatjana Grdanoska; Elena Trajkovska-Dokic
Journal:  Open Access Maced J Med Sci       Date:  2019-06-30

4.  Low-toxin Clostridioides difficile RT027 strains exhibit robust virulence.

Authors:  Farhan Anwar; Bryan Angelo P Roxas; Kareem W Shehab; Neil M Ampel; V K Viswanathan; Gayatri Vedantam
Journal:  Emerg Microbes Infect       Date:  2022-12       Impact factor: 19.568

5.  Recombination drives evolution of the Clostridium difficile 16S-23S rRNA intergenic spacer region.

Authors:  Sandra Janezic; Alexander Indra; Thomas Rattei; Thomas Weinmaier; Maja Rupnik
Journal:  PLoS One       Date:  2014-09-15       Impact factor: 3.240

  5 in total

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