Three-dimensional honeycomb-patterned chitosan/poly(L-lactic acid) scaffolds with improved mechanical and cell compatibility.

Micro-size patterned surfaces trigger specific biological responses such as the promotion of cell growth, cell migration, cell differentiation, and ECM production. The aim of this work was to elaborate three-dimensional scaffolds with honeycomb patterned surfaces and large open pores, and to study the influence of surface patterning on cell behavior. In this study, we used water droplets as porogen material to prepare a novel type of chitosan sponge with large open pores on its surface. The sponges obtained were then immersed into 6 wt % Poly(L-lactic acid) chloroform solution to obtain honeycomb patterned composite porous scaffolds. The morphology and mechanical properties were characterized with SEM and compression testing. The fibroblast behaviors in scaffolds were analyzed with SEM, VG, PAS, live-dead staining, and flow cytometer. Results showed that these composite scaffolds possessed better mechanical properties and hierarchical porous structure than pure chitosan sponges. Cell culture revealed that the honeycomb patterned surface had positive influences on fibroblast behaviors, wherein the cell adhesion, proliferation, ECM secretion and viability were improved dramatically. Such a hierarchical composite scaffold would be a suitable candidate for tissue engineering purposes.