OBJECTIVES: To evaluate the performances of an allergen microarray in multi-sensitized allergic patients with respiratory symptoms. DESIGN AND METHODS: 321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and results were compared with extract-based ImmunoCAP Allergens sIgE to 15 common airborne allergens. RESULTS: The reproducibility of ISAC was good. The Positive Percent Agreement (PPA) varied between 75% and 100% for sIgE levels above 1 kUA/l. For samples with sIgE levels below 0.1 kUA/l, the Negative Percent Agreement (NPA) ranged between 90% and 100%. Notably, 58% of respiratory allergy patients had IgE to food-specific proteins and 52% resulted sensitized to cross-reactive pan-allergens. CONCLUSION: ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules.
OBJECTIVES: To evaluate the performances of an allergen microarray in multi-sensitized allergicpatients with respiratory symptoms. DESIGN AND METHODS: 321 patients and 92 controls were included in this study. Specific serum IgE were assayed using ImmunoCAP ISAC, a microarray containing 103 components derived from 47 allergens and results were compared with extract-based ImmunoCAP Allergens sIgE to 15 common airborne allergens. RESULTS: The reproducibility of ISAC was good. The Positive Percent Agreement (PPA) varied between 75% and 100% for sIgE levels above 1 kUA/l. For samples with sIgE levels below 0.1 kUA/l, the Negative Percent Agreement (NPA) ranged between 90% and 100%. Notably, 58% of respiratory allergypatients had IgE to food-specific proteins and 52% resulted sensitized to cross-reactive pan-allergens. CONCLUSION: ImmunoCAP ISAC detects allergen sensitization at component level and adds important information by defining both cross and co-sensitization to a large variety of allergen molecules.
Authors: Ignacio J Ansotegui; Giovanni Melioli; Giorgio Walter Canonica; Luis Caraballo; Elisa Villa; Motohiro Ebisawa; Giovanni Passalacqua; Eleonora Savi; Didier Ebo; R Maximiliano Gómez; Olga Luengo Sánchez; John J Oppenheimer; Erika Jensen-Jarolim; David A Fischer; Tari Haahtela; Martti Antila; Jean J Bousquet; Victoria Cardona; Wen Chin Chiang; Pascal M Demoly; Lawrence M DuBuske; Marta Ferrer Puga; Roy Gerth van Wijk; Sandra Nora González Díaz; Alexei Gonzalez-Estrada; Edgardo Jares; Ayse Füsun Kalpaklioğlu; Luciana Kase Tanno; Marek L Kowalski; Dennis K Ledford; Olga Patricia Monge Ortega; Mário Morais Almeida; Oliver Pfaar; Lars K Poulsen; Ruby Pawankar; Harald E Renz; Antonino G Romano; Nelson A Rosário Filho; Lanny Rosenwasser; Mario A Sánchez Borges; Enrico Scala; Gian-Enrico Senna; Juan Carlos Sisul; Mimi L K Tang; Bernard Yu-Hor Thong; Rudolf Valenta; Robert A Wood; Torsten Zuberbier Journal: World Allergy Organ J Date: 2020-02-25 Impact factor: 4.084
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Authors: Christian Lupinek; Eva Wollmann; Alexandra Baar; Srinita Banerjee; Heimo Breiteneder; Barbara M Broecker; Merima Bublin; Mirela Curin; Sabine Flicker; Tetiana Garmatiuk; Heidrun Hochwallner; Irene Mittermann; Sandra Pahr; Yvonne Resch; Kenneth H Roux; Bharani Srinivasan; Sebastian Stentzel; Susanne Vrtala; Leanna N Willison; Magnus Wickman; Karin C Lødrup-Carlsen; Josep Maria Antó; Jean Bousquet; Claus Bachert; Daniel Ebner; Thomas Schlederer; Christian Harwanegg; Rudolf Valenta Journal: Methods Date: 2013-10-22 Impact factor: 3.608
Authors: Johannes Huss-Marp; Jan Gutermuth; Ina Schäffner; Ulf Darsow; Florian Pfab; Knut Brockow; Johannes Ring; Heidrun Behrendt; Thilo Jakob; Christoph Ahlgrim Journal: Allergo J Int Date: 2015-03-14