PURPOSE: Electron beam (e-beam) efficiently and non-thermally inactivates microorganisms in food by lethal DNA changes (direct effects) and free radicals from water radiolysis (in-direct effects). Non-pathogenic Escherichia coli DH5α (α substrain of DH5 described by Hanahan 1985 , 'DH' stands for Douglas Hanahan) is a microorganism that lacks DNA repair capability, resulting in high radiosensitivity. Studying microbial inactivation of E. coli DH5α repeatedly subjected to sub-lethal e-beam in ground beef may enhance understanding of microbial radioresistance. The objective of this study was to determine if repetitive processing with e-beam at sub-lethal doses increases D-value (e-beam dose required to inactivate one log of microbial population) of E. coli DH5α in ground beef. MATERIALS AND METHODS: Survivors from the highest e-beam dose were isolated and incubated in ground beef for the next cycle of e-beam processing. Five cycles were conducted. To acclimatise E. coli DH5α, first two cycles used low doses. D-values were determined following the third cycle. RESULTS: D-values increased (p < 0.05) significantly with each cycle. Thus, E. coli DH5α has a capability to develop greater radioresistance under these experimental conditions. Following the third cycle D-values were 0.32 ± 0.006 and 0.32 ± 0.002 kGy for survivors enumerated on non-selective and selective media, respectively; the fourth cycle 0.39 ± 0.007 and 0.40 ± 0.019 kGy; and the fifth cycle 0.46 ± 0.006 and 0.46 ± 0.020 kGy. D-values on non-selective and selective media were similar (p > 0.05) indicating absence of cell recovery in E. coli DH5α. CONCLUSIONS: E. coli DH5α increases radioresistance to e-beam as a result of repetitive exposure to sub-lethal doses despite its DNA repair deficiency.
PURPOSE: Electron beam (e-beam) efficiently and non-thermally inactivates microorganisms in food by lethal DNA changes (direct effects) and free radicals from water radiolysis (in-direct effects). Non-pathogenic Escherichia coli DH5α (α substrain of DH5 described by Hanahan 1985 , 'DH' stands for Douglas Hanahan) is a microorganism that lacks DNA repair capability, resulting in high radiosensitivity. Studying microbial inactivation of E. coli DH5α repeatedly subjected to sub-lethal e-beam in ground beef may enhance understanding of microbial radioresistance. The objective of this study was to determine if repetitive processing with e-beam at sub-lethal doses increases D-value (e-beam dose required to inactivate one log of microbial population) of E. coli DH5α in ground beef. MATERIALS AND METHODS: Survivors from the highest e-beam dose were isolated and incubated in ground beef for the next cycle of e-beam processing. Five cycles were conducted. To acclimatise E. coli DH5α, first two cycles used low doses. D-values were determined following the third cycle. RESULTS: D-values increased (p < 0.05) significantly with each cycle. Thus, E. coli DH5α has a capability to develop greater radioresistance under these experimental conditions. Following the third cycle D-values were 0.32 ± 0.006 and 0.32 ± 0.002 kGy for survivors enumerated on non-selective and selective media, respectively; the fourth cycle 0.39 ± 0.007 and 0.40 ± 0.019 kGy; and the fifth cycle 0.46 ± 0.006 and 0.46 ± 0.020 kGy. D-values on non-selective and selective media were similar (p > 0.05) indicating absence of cell recovery in E. coli DH5α. CONCLUSIONS: E. coli DH5α increases radioresistance to e-beam as a result of repetitive exposure to sub-lethal doses despite its DNA repair deficiency.
Authors: Vladimir S Cheptsov; Elena A Vorobyova; Natalia A Manucharova; Mikhail V Gorlenko; Anatoli K Pavlov; Maria A Vdovina; Vladimir N Lomasov; Sergey A Bulat Journal: Extremophiles Date: 2017-10-09 Impact factor: 2.395
Authors: Wiebke Tebrün; Gerzon Motola; Mohamed Hafez Hafez; Josef Bachmeier; Volker Schmidt; Kevin Renfert; Christian Reichelt; Sarah Brüggemann-Schwarze; Michael Pees Journal: PLoS One Date: 2020-05-14 Impact factor: 3.240