Literature DB >> 21625685

A luminescent oxygen channeling biosensor that measures small GTPase activation.

Björn Niebel1, Benjamin Weiche, Alan L Mueller, Dean Y Li, Nora Karnowski, Michael Famulok.   

Abstract

We established a homogeneous luminescent oxygen channeling sensor for measuring activation states of small GTPases. The assay quantifies activated GTPases in cell lysates, can be applied to different GTPases, and can be used for multiplex screening. The study will provide guidelines for determining activation states of diverse GTPases in various biological contexts.

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Year:  2011        PMID： 21625685      PMCID： PMC3961041          DOI： 10.1039/c1cc11944c

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  40 in total

Review 1.  Regulators and effectors of the ARF GTPases.

Authors:  J G Donaldson; C L Jackson
Journal:  Curr Opin Cell Biol       Date:  2000-08       Impact factor: 8.382

2.  A homogeneous 384-well high-throughput binding assay for a TNF receptor using alphascreen technology.

Authors:  Janet Wilson; Claudia Pena Rossi; Susanna Carboni; Christèle Fremaux; Dominique Perrin; Claudio Soto; Marie Kosco-Vilbois; Alexander Scheer
Journal:  J Biomol Screen       Date:  2003-10

3.  Rational design and characterization of a Rac GTPase-specific small molecule inhibitor.

Authors:  Yuan Gao; J Bradley Dickerson; Fukun Guo; Jie Zheng; Yi Zheng
Journal:  Proc Natl Acad Sci U S A       Date:  2004-05-05       Impact factor: 11.205

4.  AlphaScreen kinase HTS platforms.

Authors:  Greg Warner; Chantal Illy; Liliana Pedro; Philippe Roby; Roger Bossé
Journal:  Curr Med Chem       Date:  2004-03       Impact factor: 4.530

5.  RalGDS-like factor (Rlf) is a novel Ras and Rap 1A-associating protein.

Authors:  R M Wolthuis; B Bauer; L J van 't Veer; A M de Vries-Smits; R H Cool; M Spaargaren; A Wittinghofer; B M Burgering; J L Bos
Journal:  Oncogene       Date:  1996-07-18       Impact factor: 9.867

6.  Luminescent oxygen channeling immunoassay: measurement of particle binding kinetics by chemiluminescence.

Authors:  E F Ullman; H Kirakossian; S Singh; Z P Wu; B R Irvin; J S Pease; A C Switchenko; J D Irvine; A Dafforn; C N Skold
Journal:  Proc Natl Acad Sci U S A       Date:  1994-06-07       Impact factor: 11.205

7.  Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.

Authors:  Alexander Gray; Henric Olsson; Ian H Batty; Larisa Priganica; C Peter Downes
Journal:  Anal Biochem       Date:  2003-02-15       Impact factor: 3.365

Review 8.  The ghost in the machine: small GTPases as spatial regulators of exocytosis.

Authors:  Hao Wu; Guendalina Rossi; Patrick Brennwald
Journal:  Trends Cell Biol       Date:  2008-08-14       Impact factor: 20.808

9.  High throughput screening technologies for direct cyclic AMP measurement.

Authors:  Daniela Gabriel; Magali Vernier; Martin J Pfeifer; Boris Dasen; Laurent Tenaillon; Rochdi Bouhelal
Journal:  Assay Drug Dev Technol       Date:  2003-04       Impact factor: 1.738

10.  Overexpression of active Syrian golden hamster prion protein PrPc as a glutathione S-transferase fusion in heterologous systems.

Authors:  S Weiss; M Famulok; F Edenhofer; Y H Wang; I M Jones; M Groschup; E L Winnacker
Journal:  J Virol       Date:  1995-08       Impact factor: 5.103
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