Literature DB >> 2162520

Amplification of genomic sequences flanking transposable elements in host and heterologous plants: a tool for transposon tagging and genome characterization.

D J Earp1, B Lowe, B Baker.   

Abstract

The isolation of sequences flanking integrated transposable elements is an important step in gene tagging strategies. We have demonstrated that sequences flanking transposons integrated into complex genomes can be simply and rapidly obtained using the polymerase chain reaction. Amplification of such sequences was established in a model system, a transgenic tobacco plant carrying a single Ac element, and successfully applied to the cloning of a specific Spm element from a maize line carrying multiple Spm hybridizing sequences. The described utilization of methylation sensitive restriction enzymes (including those with degenerate recognition sequences) in the generation of templates for amplification will simplify the cloning and mapping of genomic sequences adjacent to transposable elements.

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Year:  1990        PMID： 2162520      PMCID： PMC330933          DOI： 10.1093/nar/18.11.3271

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  40 in total

1.  A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences.

Authors:  T Triglia; M G Peterson; D J Kemp
Journal:  Nucleic Acids Res       Date:  1988-08-25       Impact factor: 16.971

2.  Transposition of Ac from the P locus of maize into unreplicated chromosomal sites.

Authors:  J Chen; I M Greenblatt; S L Dellaporta
Journal:  Genetics       Date:  1987-09       Impact factor: 4.562

3.  EcoK selection vectors for shotgun cloning into M13 and deletion mutagenesis.

Authors:  M M Waye; M E Verhoeyen; P T Jones; G Winter
Journal:  Nucleic Acids Res       Date:  1985-12-09       Impact factor: 16.971

4.  Molecular genetic analysis of the dilute-short ear (d-se) region of the mouse.

Authors:  E M Rinchik; L B Russell; N G Copeland; N A Jenkins
Journal:  Genetics       Date:  1986-02       Impact factor: 4.562

5.  The frequency of transposition of the maize element Activator is not affected by an adjacent deletion.

Authors:  H K Dooner; J English; E J Ralston
Journal:  Mol Gen Genet       Date:  1988-03

6.  Isolation of the transposable maize controlling elements Ac and Ds.

Authors:  N Fedoroff; S Wessler; M Shure
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

7.  Cloning of DNA sequences from the white locus of D. melanogaster by a novel and general method.

Authors:  P M Bingham; R Levis; G M Rubin
Journal:  Cell       Date:  1981-09       Impact factor: 41.582

8.  Molecular cloning of the c locus of Zea mays: a locus regulating the anthocyanin pathway.

Authors:  J Paz-Ares; U Wienand; P A Peterson; H Saedler
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

9.  Molecular analysis of the En/Spm transposable element system of Zea mays.

Authors:  A Pereira; H Cuypers; A Gierl; Z Schwarz-Sommer; H Saedler
Journal:  EMBO J       Date:  1986-05       Impact factor: 11.598

10.  Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator.

Authors:  R A Martienssen; A Barkan; M Freeling; W C Taylor
Journal:  EMBO J       Date:  1989-06       Impact factor: 11.598
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  19 in total

1.  Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis.

Authors:  R Greco; P B Ouwerkerk; A J Taal; C Favalli; T Beguiristain; P Puigdomènech; L Colombo; J H Hoge; A Pereira
Journal:  Plant Mol Biol       Date:  2001-05       Impact factor: 4.076

2.  Use of the transposon Ac as a gene-searching engine in the maize genome.

Authors:  Matthew Cowperthwaite; Wonkeun Park; Zhennan Xu; Xianghe Yan; Steven C Maurais; Hugo K Dooner
Journal:  Plant Cell       Date:  2002-03       Impact factor: 11.277

3.  Characterization of the Ac/Ds behaviour in transgenic tomato plants using plasmid rescue.

Authors:  C M Rommens; G N Rudenko; P P Dijkwel; M J van Haaren; P B Ouwerkerk; K M Blok; H J Nijkamp; J Hille
Journal:  Plant Mol Biol       Date:  1992-10       Impact factor: 4.076

4.  Perspectives on Genetic Analysis of Plant Embryogenesis.

Authors:  D. W. Meinke
Journal:  Plant Cell       Date:  1991-09       Impact factor: 11.277

5.  Transcription and somatic transposition of the maize En/Spm transposon system in rice.

Authors:  R Greco; P B F Ouwerkerk; A J C Taal; C Sallaud; E Guiderdoni; A H Meijer; J H C Hoge; A Pereira
Journal:  Mol Genet Genomics       Date:  2003-11-14       Impact factor: 3.291

6.  Ac transposition from a T-DNA can generate linked and unlinked clusters of insertions in the tomato genome.

Authors:  B I Osborne; C A Corr; J P Prince; R Hehl; S D Tanksley; S McCormick; B Baker
Journal:  Genetics       Date:  1991-11       Impact factor: 4.562

Review 7.  The use of transgenic plants to understand transposition mechanisms and to develop transposon tagging strategies.

Authors:  M A Haring; C M Rommens; H J Nijkamp; J Hille
Journal:  Plant Mol Biol       Date:  1991-03       Impact factor: 4.076

8.  Transposition of the maize activator element in transgenic rice plants.

Authors:  N Murai; Z J Li; Y Kawagoe; A Hayashimoto
Journal:  Nucleic Acids Res       Date:  1991-02-11       Impact factor: 16.971

9.  Direct haplotype determination by double ARMS: specificity, sensitivity and genetic applications.

Authors:  Y M Lo; P Patel; C R Newton; A F Markham; K A Fleming; J S Wainscoat
Journal:  Nucleic Acids Res       Date:  1991-07-11       Impact factor: 16.971

10.  RPE, a plant gene involved in early developmental steps of nematode feeding cells.

Authors:  B Favery; P Lecomte; N Gil; N Bechtold; D Bouchez; A Dalmasso; P Abad
Journal:  EMBO J       Date:  1998-12-01       Impact factor: 11.598
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