Literature DB >> 2162518

Fast solid support detection of PCR amplified viral DNA sequences using radioiodinated or hapten labelled primers.

S Sauvaigo1, B Fouqué, A Roget, T Livache, H Bazin, C Chypre, R Téoule.   

Abstract

Oligonucleotides with novel modifications have been synthesized and incorporated into enzymatically amplified DNA sequences. They allow the fast detection of viral DNA sequences after two rounds of amplification. The hybrids formed are immobilized by affinity on coated tubes and detected by direct beta (32P) or gamma (125I) counting or by colorimetric revelation. The effect of a dilution step between the two amplifications is studied to obtain optimal sensitivity and specificity. This test is used to detect Human Papillomavirus types 16 and 18 in cells and biopsies and for the specific colorimetric detection of HIV1 in extracted DNA.

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Year:  1990        PMID： 2162518      PMCID： PMC330921          DOI： 10.1093/nar/18.11.3175

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors:  F C GREENWOOD; W M HUNTER; J S GLOVER
Journal:  Biochem J       Date:  1963-10       Impact factor: 3.857

2.  A method for gene enrichment based on the avidin-biotin interaction. Application to the Drosophila ribosomal RNA genes.

Authors:  J Manning; M Pellegrini; N Davidson
Journal:  Biochemistry       Date:  1977-04-05       Impact factor: 3.162

3.  Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.

Authors:  R K Saiki; S Scharf; F Faloona; K B Mullis; G T Horn; H A Erlich; N Arnheim
Journal:  Science       Date:  1985-12-20       Impact factor: 47.728

4.  A simple and efficient enzymatic method for covalent attachment of DNA to cellulose. Application for hybridization-restriction analysis and for in vitro synthesis of DNA probes.

Authors:  T Goldkorn; D J Prockop
Journal:  Nucleic Acids Res       Date:  1986-11-25       Impact factor: 16.971

5.  DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.

Authors:  C Y Ou; S Kwok; S W Mitchell; D H Mack; J J Sninsky; J W Krebs; P Feorino; D Warfield; G Schochetman
Journal:  Science       Date:  1988-01-15       Impact factor: 47.728

6.  Direct cloning and sequence analysis of enzymatically amplified genomic sequences.

Authors:  S J Scharf; G T Horn; H A Erlich
Journal:  Science       Date:  1986-09-05       Impact factor: 47.728

7.  Iodination of nucleic acids in vitro.

Authors:  S L Commerford
Journal:  Biochemistry       Date:  1971-05-25       Impact factor: 3.162

8.  Fast quantification of nucleic acid hybrids by affinity-based hybrid collection.

Authors:  A C Syvänen; M Laaksonen; H Söderlund
Journal:  Nucleic Acids Res       Date:  1986-06-25       Impact factor: 16.971

9.  The final deprotection step in oligonucleotide synthesis is reduced to a mild and rapid ammonia treatment by using labile base-protecting groups.

Authors:  J C Schulhof; D Molko; R Teoule
Journal:  Nucleic Acids Res       Date:  1987-01-26       Impact factor: 16.971

10.  A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.

Authors:  M Schwab; K Alitalo; H E Varmus; J M Bishop; D George
Journal:  Nature       Date:  1983 Jun 9-15       Impact factor: 49.962
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  6 in total

1.  Solid support quantitation of c-myc PCR products using a cleavable reporter.

Authors:  S Rhoer-Moja; H Bazin; S Sauvaigo; C Chypre; M Vindimian
Journal:  Nucleic Acids Res       Date:  1994-02-11       Impact factor: 16.971

2.  The production of PCR products with 5' single-stranded tails using primers that incorporate novel phosphoramidite intermediates.

Authors:  C R Newton; D Holland; L E Heptinstall; I Hodgson; M D Edge; A F Markham; M J McLean
Journal:  Nucleic Acids Res       Date:  1993-03-11       Impact factor: 16.971

3.  Standardized nested polymerase chain reaction-based assay for detection of human immunodeficiency virus type 1 DNA in whole blood lysates.

Authors:  S Sauvaigo; V Barlet; N Guettari; P Innocenti; F Parmentier; C Bastard; J M Seigneurin; J C Chermann; R Teoule; J Marchand
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

4.  Drug targeting: synthesis and endocytosis of oligonucleotide-neoglycoprotein conjugates.

Authors:  E Bonfils; C Depierreux; P Midoux; N T Thuong; M Monsigny; A C Roche
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

5.  Enzyme-linked oligosorbent assay for detection of polymerase chain reaction-amplified human immunodeficiency virus type 1.

Authors:  F Mallet; C Hebrard; D Brand; E Chapuis; P Cros; P Allibert; J M Besnier; F Barin; B Mandrand
Journal:  J Clin Microbiol       Date:  1993-06       Impact factor: 5.948

6.  Biodistribution of iodine-125 tyramine transforming growth factor alpha antisense oligonucleotide in athymic mice with a human mammary tumour xenograft following intratumoral injection.

Authors:  S Cammilleri; S Sangrajrang; B Perdereau; F Brixy; F Calvo; H Bazin; H Magdelenat
Journal:  Eur J Nucl Med       Date:  1996-04
  6 in total

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