OBJECTIVES: To conduct a pilot study to investigate functional, metabolic, and penile morphologic changes in a novel model of lean DM2. Erectile dysfunction (ED) is a frequent sequela in patients with type 2 diabetes mellitus (DM2). METHODS: Eight rats received a high-fat diet and 2 weeks later, 2 intraperitoneal injections of streptozotocin (STZ, 30 mg/kg). Five age-matched rats served as controls. Insulin challenge tests were performed at 6 and 12 weeks after induction of DM2. At 12 weeks, erectile function was tested by measurement of intracavernous pressure (ICP) increase upon cavernous nerve stimulation. Penile tissue and serum samples were harvested for histology and biochemistry, respectively. RESULTS: A lean DM2 model was established as demonstrated by decreased insulin resistance, elevated nonfasting plasma glucose levels, hyperlipidemia, and decreased insulin concentration in the absence of obesity. ICP/mean arterial pressure was significantly decreased in DM2 animals (0.29) compared with controls (0.81). Expression of neuronal nitric oxide synthase and rat endothelial cell antigen-1, and the smooth muscle/collagen ratio were significantly decreased in the penis of DM2 animals. CONCLUSIONS: We propose an inexpensive nongenetic animal model of lean DM2-associated ED. Microanatomical changes in the erectile tissue that reflect an advanced stage of the disease were observed.
OBJECTIVES: To conduct a pilot study to investigate functional, metabolic, and penile morphologic changes in a novel model of lean DM2. Erectile dysfunction (ED) is a frequent sequela in patients with type 2 diabetes mellitus (DM2). METHODS: Eight rats received a high-fat diet and 2 weeks later, 2 intraperitoneal injections of streptozotocin (STZ, 30 mg/kg). Five age-matched rats served as controls. Insulin challenge tests were performed at 6 and 12 weeks after induction of DM2. At 12 weeks, erectile function was tested by measurement of intracavernous pressure (ICP) increase upon cavernous nerve stimulation. Penile tissue and serum samples were harvested for histology and biochemistry, respectively. RESULTS: A lean DM2 model was established as demonstrated by decreased insulin resistance, elevated nonfasting plasma glucose levels, hyperlipidemia, and decreased insulin concentration in the absence of obesity. ICP/mean arterial pressure was significantly decreased in DM2 animals (0.29) compared with controls (0.81). Expression of neuronal nitric oxide synthase and rat endothelial cell antigen-1, and the smooth muscle/collagen ratio were significantly decreased in the penis of DM2 animals. CONCLUSIONS: We propose an inexpensive nongenetic animal model of lean DM2-associated ED. Microanatomical changes in the erectile tissue that reflect an advanced stage of the disease were observed.
Authors: K Otero; F Martínez; A Beltrán; D González; B Herrera; G Quintero; R Delgado; A Rojas Journal: Biochem J Date: 2001-11-01 Impact factor: 3.857
Authors: Hotaka Matsui; Biljana Musicki; Nikolai A Sopko; Xiaopu Liu; Paula J Hurley; Arthur L Burnett; Trinity J Bivalacqua; Johanna L Hannan Journal: Urology Date: 2016-09-14 Impact factor: 2.649
Authors: Guiting Lin; Xuefeng Qiu; Thomas M Fandel; Maarten Albersen; Zhong Wang; Tom F Lue; Ching-Shwun Lin Journal: Urology Date: 2011-08-16 Impact factor: 2.649
Authors: Carol A Podlasek; John Mulhall; Kelvin Davies; Christopher J Wingard; Johanna L Hannan; Trinity J Bivalacqua; Biljana Musicki; Mohit Khera; Nestor F González-Cadavid; Arthur L Burnett Journal: J Sex Med Date: 2016-08 Impact factor: 3.802
Authors: Xuefeng Qiu; Guiting Lin; Zhongcheng Xin; Ludovic Ferretti; Haiyang Zhang; Tom F Lue; Ching-Shwun Lin Journal: J Sex Med Date: 2012-12-17 Impact factor: 3.802