OBJECTIVE: To confirm a microarray study that suggested that Kindlin-2 might play a role in the development and progression of bladder cancer. There has been no previous examination of Kindlin-2 expression in human bladder cancer. METHODS: A combination of real-time polymerase chain reaction, Western analysis, and immunohistochemistry was used to characterize Kindlin-2 expression in arsenite (As(+3))- and cadmium (Cd(+2))-transformed human cell lines, their tumor transplants in immunocompromised mice, and in archival specimens of human bladder and bladder cancer. RESULTS: The results show that the Kindlin-2 expression patterns in the cell lines were not duplicated in the tumor tissues. However, it was shown that Kindlin-2 was expressed in the stromal element of all the transplanted tumors and archival specimens of human bladder cancer. It was also shown that a small number of high-grade invasive urothelial cancers have focal expression of Kindlin-2 in the tumor cells. CONCLUSION: Kindlin-2 is expressed in the stromal component of most, if not all, human bladder cancers. Kindlin-2 is not expressed in normal urothelium. Kindlin-2 is expressed in a small subset of high-grade invasive bladder cancers and may have potential as a prognostic marker for tumor progression.
OBJECTIVE: To confirm a microarray study that suggested that Kindlin-2 might play a role in the development and progression of bladder cancer. There has been no previous examination of Kindlin-2 expression in humanbladder cancer. METHODS: A combination of real-time polymerase chain reaction, Western analysis, and immunohistochemistry was used to characterize Kindlin-2 expression in arsenite (As(+3))- and cadmium (Cd(+2))-transformed human cell lines, their tumor transplants in immunocompromised mice, and in archival specimens of humanbladder and bladder cancer. RESULTS: The results show that the Kindlin-2 expression patterns in the cell lines were not duplicated in the tumor tissues. However, it was shown that Kindlin-2 was expressed in the stromal element of all the transplanted tumors and archival specimens of humanbladder cancer. It was also shown that a small number of high-grade invasive urothelial cancers have focal expression of Kindlin-2 in the tumor cells. CONCLUSION:Kindlin-2 is expressed in the stromal component of most, if not all, humanbladder cancers. Kindlin-2 is not expressed in normal urothelium. Kindlin-2 is expressed in a small subset of high-grade invasive bladder cancers and may have potential as a prognostic marker for tumor progression.
Authors: Ling Cao; Xu Dong Zhou; Mary Ann Sens; Scott H Garrett; Yun Zheng; Jane R Dunlevy; Donald A Sens; Seema Somji Journal: J Appl Toxicol Date: 2010-07 Impact factor: 3.446
Authors: Donald A Sens; Seongmi Park; Volkan Gurel; Mary Ann Sens; Scott H Garrett; Seema Somji Journal: Toxicol Sci Date: 2004-02-19 Impact factor: 4.849
Authors: T Tsuda; A Babazono; E Yamamoto; N Kurumatani; Y Mino; T Ogawa; Y Kishi; H Aoyama Journal: Am J Epidemiol Date: 1995-02-01 Impact factor: 4.897
Authors: H Y Chiou; Y M Hsueh; K F Liaw; S F Horng; M H Chiang; Y S Pu; J S Lin; C H Huang; C J Chen Journal: Cancer Res Date: 1995-03-15 Impact factor: 12.701
Authors: M R Rossi; J R Masters; S Park; J H Todd; S H Garrett; M A Sens; S Somji; J Nath; D A Sens Journal: Environ Health Perspect Date: 2001-08 Impact factor: 9.031