Literature DB >> 2162321

Phytase from Klebsiella Sp. No. PG-2: purification and properties.

V Shah1, L J Parekh.   

Abstract

A phytase (EC 3.1.3.8) was extracted from rat intestinal bacterium, Klebsiella Sp. No. PG.-2, and purified 50-fold by ammonium sulphate fractionation, ion-exchange chromatography and gel filtration. The enzyme is inducible in nature. The pH optimum was at 6.0 for all the inositol phosphates studied and this characterized the enzyme as an acid phosphohydrolase. Of a range of potential substrates tested, only p-nitrophenyl phosphate alongwith the inositol phosphates was hydrolyzed. It exhibits a Km of 2.0 mM; temperature optimum of 37 degrees C and energy of activation 9,120 cal/mole for all the inositol phosphates studied. The activity was inhibited by Ag2+, Hg2+, Cu2+, fluoride and high substrate concentration.

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Year:  1990        PMID： 2162321

Source DB:  PubMed          Journal:  Indian J Biochem Biophys        ISSN： 0301-1208            Impact factor:   1.918


  7 in total

1.  Isolation and characterization of a novel phytase from Penicillium simplicissimum.

Authors:  Y H Tseng; T J Fang; S M Tseng
Journal:  Folia Microbiol (Praha)       Date:  2000       Impact factor: 2.099

2.  Biochemical characterisation of extracellular phytase (myo-inositol hexakisphosphate phosphohydrolase) from a hyper-producing strain of Aspergillus niger van Teighem.

Authors:  Purva Vats; U C Banerjee
Journal:  J Ind Microbiol Biotechnol       Date:  2005-03-18       Impact factor: 3.346

Review 3.  Phytase: sources, preparation and exploitation.

Authors:  J Dvoráková
Journal:  Folia Microbiol (Praha)       Date:  1998       Impact factor: 2.099

4.  Analysis of myo-inositol hexakisphosphate hydrolysis by Bacillus phytase: indication of a novel reaction mechanism.

Authors:  J Kerovuo; J Rouvinen; F Hatzack
Journal:  Biochem J       Date:  2000-12-15       Impact factor: 3.857

5.  Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis.

Authors:  J Kerovuo; M Lauraeus; P Nurminen; N Kalkkinen; J Apajalahti
Journal:  Appl Environ Microbiol       Date:  1998-06       Impact factor: 4.792

6.  Phytate-degrading enzyme production by bacteria isolated from Malaysian soil.

Authors:  Anis Shobirin Meor Hussin; Abd-ElAziem Farouk; Ralf Greiner; Hamzah Mohd Salleh; Ahmad Faris Ismail
Journal:  World J Microbiol Biotechnol       Date:  2007-05-15       Impact factor: 3.312

7.  Novel Glucose-1-Phosphatase with High Phytase Activity and Unusual Metal Ion Activation from Soil Bacterium Pantoea sp. Strain 3.5.1.

Authors:  Aliya D Suleimanova; Astrid Beinhauer; Liia R Valeeva; Inna B Chastukhina; Nelly P Balaban; Eugene V Shakirov; Ralf Greiner; Margarita R Sharipova
Journal:  Appl Environ Microbiol       Date:  2015-07-24       Impact factor: 4.792
  7 in total

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