Theresa M Culley1, J Ryan Stewart. 1. Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221-0006 USA.
Abstract
PREMISE OF THE STUDY: Microsatellite primers were developed in the introduced shrub Rhamnus cathartica L., to investigate potential hybridization events with related taxa as a stimulus of invasive spread. • METHODS AND RESULTS: Using a non-radioactive protocol, 15 primer sets were identified in North American and European populations of R. cathartica. The primers amplified di-, tri- and pentanucelotide repeats with 1-14 alleles per locus. Most primers also amplified in R. lanceolata, R. smithii, and/or Frangula alnus (previously R. frangula). • CONCLUSIONS: These results indicate the utility of primers in R. cathartica for future genetic studies as well as their potential applicability in other related taxa.
PREMISE OF THE STUDY: Microsatellite primers were developed in the introduced shrub Rhamnus cathartica L., to investigate potential hybridization events with related taxa as a stimulus of invasive spread. • METHODS AND RESULTS: Using a non-radioactive protocol, 15 primer sets were identified in North American and European populations of R. cathartica. The primers amplified di-, tri- and pentanucelotide repeats with 1-14 alleles per locus. Most primers also amplified in R. lanceolata, R. smithii, and/or Frangula alnus (previously R. frangula). • CONCLUSIONS: These results indicate the utility of primers in R. cathartica for future genetic studies as well as their potential applicability in other related taxa.
Authors: Theresa M Culley; Trevor I Stamper; Richard L Stokes; Jessica R Brzyski; Nicole A Hardiman; Matthew R Klooster; Benjamin J Merritt Journal: Appl Plant Sci Date: 2013-10-01 Impact factor: 1.936