BACKGROUND: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. MATERIALS AND METHODS: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. RESULTS: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G(1) and increased G(2)/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. CONCLUSION: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
BACKGROUND: Deletions or mutations of the phosphatase and tensin homolog (PTEN) are frequently observed in malignant glioma and are responsible for progression of the disease. Since the molecule is a promising target for gene therapy, the effects of PTEN on glioma proliferation in combination with the anti-neoplastic agent, temozolomide, and ionizing radiation were investigated. MATERIALS AND METHODS: An adenoviral vector encoding PTEN was used. After infection, changes in proliferation, the cell cycle, as well as drug- and radiosensitivity were investigated. RESULTS: Expression of PTEN led to a 1.21-fold prolongation of the doubling time of the cells. It reduced G(1) and increased G(2)/M populations. Forced PTEN expression conferred sensitivity to temozolomide and/or ionizing radiation. CONCLUSION: In addition to counteracting cell proliferation, expression of PTEN presented advantages in the chemo- and radiosensitivity of glioma cells. Methods for up-regulation of PTEN may have a role in increasing the efficacy of current adjuvant therapies.
Authors: Mark Vitucci; Natalie O Karpinich; Ryan E Bash; Andrea M Werneke; Ralf S Schmid; Kristen K White; Robert S McNeill; Byron Huff; Sophie Wang; Terry Van Dyke; C Ryan Miller Journal: Neuro Oncol Date: 2013-06-27 Impact factor: 12.300
Authors: Charles E Rutter; Kimberly L Johung; Xiaopan Yao; Alex Y Lu; Lucia B Jilaveanu; James B Yu; Joseph N Contessa; Harriet M Kluger; Veronica L S Chiang; Ranjit S Bindra Journal: J Radiosurg SBRT Date: 2016
Authors: Kaushik Banerjee; Felipe J Núñez; Santiago Haase; Brandon L McClellan; Syed M Faisal; Stephen V Carney; Jin Yu; Mahmoud S Alghamri; Antonela S Asad; Alejandro J Nicola Candia; Maria Luisa Varela; Marianela Candolfi; Pedro R Lowenstein; Maria G Castro Journal: Front Mol Neurosci Date: 2021-03-11 Impact factor: 5.639