PREMISE OF THE STUDY: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. • METHODS AND RESULTS: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. • CONCLUSIONS: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.
PREMISE OF THE STUDY: Microsatellite primers were developed from bacterial artificial chromosome (BAC) end sequences of Citrus clementina and their transferability and polymorphism tested in the genus Citrus for future anchorage of physical and genetic maps and comparative interspecific genetic mapping. • METHODS AND RESULTS: Using PAGE and DNA silver staining, 79 primer pairs were selected for their transferability and polymorphism among 526 microsatellites mined in BES. A preliminary diversity study in Citrus was conducted with 18 of them, in C. reticulata, C. maxima, C. medica, C. sinensis, C. aurantium, C. paradisi, C. lemon, C. aurantifolia, and some papedas (wild citrus), using a capillary electrophoresis fragment analyzer. Intra- and interspecific polymorphism was observed, and heterozygous markers were identified for the different genotypes to be used for genetic mapping. • CONCLUSIONS: These results indicate the utility of the developed primers for comparative mapping studies and the integration of physical and genetic maps.
Authors: Andres Garcia-Lor; Franck Curk; Hager Snoussi-Trifa; Raphael Morillon; Gema Ancillo; François Luro; Luis Navarro; Patrick Ollitrault Journal: Ann Bot Date: 2012-10-26 Impact factor: 4.357
Authors: Patrick Ollitrault; Javier Terol; Chunxian Chen; Claire T Federici; Samia Lotfy; Isabelle Hippolyte; Frédérique Ollitrault; Aurélie Bérard; Aurélie Chauveau; Jose Cuenca; Gilles Costantino; Yildiz Kacar; Lisa Mu; Andres Garcia-Lor; Yann Froelicher; Pablo Aleza; Anne Boland; Claire Billot; Luis Navarro; François Luro; Mikeal L Roose; Frederick G Gmitter; Manuel Talon; Dominique Brunel Journal: BMC Genomics Date: 2012-11-05 Impact factor: 3.969