BACKGROUND: Adipose tissue is commonly used for volume restoration. It is also a source of adipose-derived stem cells (ASCs), easy to obtain in large quantities by liposuction or resection techniques. The aim of this study was to determine the influence of body mass index (BMI) and age on the number (yield) and proliferation capacity of ASCs. METHODS: A prospective study was conducted in 42 women. They were divided into two groups: age ≤ 40 or >40 and BMI ≤ 25 or >25. Fat tissue was harvested via manual lipoaspiration always from the abdominal region. After centrifugation in the OR, the harvested fat (100 cc) was sent to the laboratory for isolation and cultivation of ASCs. The yield of viable ASCs was evaluated by the trypan blue exclusion test. Viable ASCs were cultured and their proliferation capacity was evaluated by the growth kinetics assay. Results were statistically analyzed. RESULTS: The average cell yield was 0.380 × 10(6)/ml. Cell yield and proliferation capacity did not show statistically significant correlation to the age and BMI of patients, with regression lines showing null correlation. There was no significant difference between the cell yield and proliferation capacity between the different groups. CONCLUSION: The results from this study suggest that there is no statistically significant correlation between ASC yield and proliferation capacity and age and BMI.
BACKGROUND: Adipose tissue is commonly used for volume restoration. It is also a source of adipose-derived stem cells (ASCs), easy to obtain in large quantities by liposuction or resection techniques. The aim of this study was to determine the influence of body mass index (BMI) and age on the number (yield) and proliferation capacity of ASCs. METHODS: A prospective study was conducted in 42 women. They were divided into two groups: age ≤ 40 or >40 and BMI ≤ 25 or >25. Fat tissue was harvested via manual lipoaspiration always from the abdominal region. After centrifugation in the OR, the harvested fat (100 cc) was sent to the laboratory for isolation and cultivation of ASCs. The yield of viable ASCs was evaluated by the trypan blue exclusion test. Viable ASCs were cultured and their proliferation capacity was evaluated by the growth kinetics assay. Results were statistically analyzed. RESULTS: The average cell yield was 0.380 × 10(6)/ml. Cell yield and proliferation capacity did not show statistically significant correlation to the age and BMI of patients, with regression lines showing null correlation. There was no significant difference between the cell yield and proliferation capacity between the different groups. CONCLUSION: The results from this study suggest that there is no statistically significant correlation between ASC yield and proliferation capacity and age and BMI.
Authors: Maria Eugenia Fernández-Santos; Mariano Garcia-Arranz; Enrique J Andreu; Ana Maria García-Hernández; Miriam López-Parra; Eva Villarón; Pilar Sepúlveda; Francisco Fernández-Avilés; Damian García-Olmo; Felipe Prosper; Fermin Sánchez-Guijo; Jose M Moraleda; Agustin G Zapata Journal: Front Immunol Date: 2022-06-09 Impact factor: 8.786
Authors: Dominik Duscher; Anna Luan; Robert C Rennert; David Atashroo; Zeshaan N Maan; Elizabeth A Brett; Alexander J Whittam; Natalie Ho; Michelle Lin; Michael S Hu; Graham G Walmsley; Raphael Wenny; Manfred Schmidt; Arndt F Schilling; Hans-Günther Machens; Georg M Huemer; Derrick C Wan; Michael T Longaker; Geoffrey C Gurtner Journal: J Transl Med Date: 2016-05-06 Impact factor: 5.531
Authors: Lukas Prantl; Andreas Eigenberger; Eva Brix; Sally Kempa; Magnus Baringer; Oliver Felthaus Journal: Cells Date: 2021-05-05 Impact factor: 6.600
Authors: J-F Stoltz; N de Isla; Y P Li; D Bensoussan; L Zhang; C Huselstein; Y Chen; V Decot; J Magdalou; N Li; L Reppel; Y He Journal: Stem Cells Int Date: 2015-08-02 Impact factor: 5.443