| Literature DB >> 2161419 |
J B Molloy1, P J Walker, F C Baldock, B J Rodwell, J A Cowley.
Abstract
An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.Entities:
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Year: 1990 PMID： 2161419 DOI： 10.1016/0166-0934(90)90086-u
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014