Differential expression of serine-threonine kinase receptor-associated protein in patients with systemic lupus erythematosus.

The serine-threonine kinase receptor-associated protein (STRAP), a 39 kDa protein localized predominantly in cytoplasm, is an important inhibitor of transforming growth factor beta (TGF-ß) signaling and a regulator of cell proliferation. To investigate the application of STRAP as a novel biomarker in evaluating the pathological condition of systemic lupus erythematosus (SLE), and to determine the possible involvement of STRAP in SLE pathogenesis, the expression levels of STRAP in peripheral blood mononuclear cells (PBMC) of SLE patients were analyzed. PBMC were collected from six patients with active SLE, six with stable SLE and six healthy controls; after protein extraction and concentration determination, the samples were labeled with reagents for isobaric tagging for relative and absolute protein quantification (iTRAQ) and detected by tandem mass spectrometry. The initial proteomic analysis identified and quantified hundreds of proteins. Of these, STRAP was found to decrease more than three-fold in active SLE patients compared with healthy controls (the relative ratio was 0.291). The under-expression of STRAP in active SLE was further verified by western blot in larger independent sample sets. Clinical data analyses revealed that the levels of STRAP in SLE inversely correlated to the SLE disease activity index (SLEDAI) (r = -0.607, p < 0.05). These results indicate that the under-expression of STRAP may be a negative factor in the pathogenic process of SLE; as a result, this may also be of clinical significance as a potential condition-specific indicator of active SLE.