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Literature DB >> 2161156

Disease symptoms in transgenic tobacco induced by integrated gene VI of cauliflower mosaic virus.

Abstract

A chimeric vector (pKR 612B1) containing the neomycin phosphotransferase (APH) gene from the Tn5 transposon under the control of the gene VI promoter of cauliflower mosaic virus (CaMV) and the cloned gene VI region (SalI-BstEII) of the same virus were used to cotransform tobacco protoplasts. Using the polyethylene glycol transformation procedure, a large number of protoplasts were transformed and proved to be resistant to kanamycin (Km). Whole Km-resistant plants were regenerated and shown to contain the integrated foreign genes. DNA from transformed clones was analyzed by Southern blot hybridization, showing the presence of the Tn5-derived gene and the viral gene. Transgenic plants containing the viral gene show mild mosaic patterns and fasciation. The expression of the gene VI product was detected by immunoblots.

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Year: 1990 PMID： 2161156 DOI： 10.1007/BF00393180

Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332

16 in total

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Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

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Journal: Virology Date: 1980-04-30 Impact factor: 3.616

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Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

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Journal: Cell Date: 1980-08 Impact factor: 41.582

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Journal: Gene Date: 1985 Impact factor: 3.688

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Authors: R D Shillito; J Paszkowski; I Potrykus
Journal: Plant Cell Rep Date: 1983-10 Impact factor: 4.570

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Authors: J Schoelz; R J Shepherd; S Daubert
Journal: Mol Cell Biol Date: 1986-07 Impact factor: 4.272

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Authors: S D Daubert; J Schoelz; L Debao; R J Shepherd
Journal: J Mol Appl Genet Date: 1984

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Authors: R C Gardner; A J Howarth; P Hahn; M Brown-Luedi; R J Shepherd; J Messing
Journal: Nucleic Acids Res Date: 1981-06-25 Impact factor: 16.971

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Authors: C Xiong; S Muller; G Lebeurier; L Hirth
Journal: EMBO J Date: 1982 Impact factor: 11.598

5 in total

1. Arabidopsis thaliana expressing the cauliflower mosaic virus ORF VI transgene has a late flowering phenotype.

Authors: C Zijlstra; N Schärer-Hernández; S Gal; T Hohn
Journal: Virus Genes Date: 1996 Impact factor: 2.332

2. Expression of a plant viral polycistronic mRNA in yeast, Saccharomyces cerevisiae, mediated by a plant virus translational transactivator.

Authors: Y Sha; E P Broglio; J F Cannon; J E Schoelz
Journal: Proc Natl Acad Sci U S A Date: 1995-09-12 Impact factor: 11.205