| Literature DB >> 21607861 |
Adriana Caballero1, Adriano Marchese.
Abstract
In this chapter, we describe a method for detecting the ubiquitination status of G protein-coupled receptors (GPCRs). This involves co-expression of a GPCR with an epitope-tagged ubiquitin construct in a -heterologous mammalian expression system. Stimulus-dependent modification of the GPCR by -ubiquitin is detected by immunoprecipitation and subsequent immunoblotting to detect incorporation of the epitope-tagged ubiquitin. We describe here a well-established protocol to detect ubiquitination of the chemokine receptor CXCR4, which can be easily applied to detect the ubiquitination status of other GPCRs.Entities:
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Year: 2011 PMID： 21607861 PMCID： PMC4699430 DOI： 10.1007/978-1-61779-126-0_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745