Baoping Song1, Bin Zhuge, Huiying Fang, Jian Zhuge. 1. The Key Laboratory of Industrial Biotechnology, Ministry of Education and the Research Centre of Industrial Microbiology, Jiangnan University, Wuxi 214122, China. bpsong711@yahoo.cn
Abstract
OBJECTIVE: To determine the chromosome DNA ploidy of Candida glycerinogenes. METHODS: We screened the haploid cell of Saccharomyces cerevisiae and the diploid of Candida tropicalis as the reference cell to identify the ploidy of Candida glycerinogenes. The concentrations of chromosome DNA extracted from both haploid and diploid cells were determined by the diphenylamine reaction method. Meanwhile, the cell number of Candida tropicalis, Candida glycerinogenes, Saccharomyces cerevisiae and its haploid were accounted by hemocytometer measurement method, respectively. Because in the same UV irradiation conditions,the haploid cells was more easy died than diploid cells, so we used the UV irradiation test to further verification. RESULTS: The results of the content of chromosome DNA in a single cell showed that Candida glycerinogenes was a diploid cell. Moreover, it was also further confirmed because the haploid cell of Saccharomyces cerevisiae was more sensitive than Candida glycerinogenes under UV treatment. CONCLUSION: By this study, we confirmed that Candida glycerinogenes was a diploid cell.
OBJECTIVE: To determine the chromosome DNA ploidy of Candida glycerinogenes. METHODS: We screened the haploid cell of Saccharomyces cerevisiae and the diploid of Candida tropicalis as the reference cell to identify the ploidy of Candida glycerinogenes. The concentrations of chromosome DNA extracted from both haploid and diploid cells were determined by the diphenylamine reaction method. Meanwhile, the cell number of Candida tropicalis, Candida glycerinogenes, Saccharomyces cerevisiae and its haploid were accounted by hemocytometer measurement method, respectively. Because in the same UV irradiation conditions,the haploid cells was more easy died than diploid cells, so we used the UV irradiation test to further verification. RESULTS: The results of the content of chromosome DNA in a single cell showed that Candida glycerinogenes was a diploid cell. Moreover, it was also further confirmed because the haploid cell of Saccharomyces cerevisiae was more sensitive than Candida glycerinogenes under UV treatment. CONCLUSION: By this study, we confirmed that Candida glycerinogenes was a diploid cell.