| Literature DB >> 2160038 |
R H Joho1, J R Moorman, A M VanDongen, G E Kirsch, H Silberberg, G Schuster, A M Brown.
Abstract
Sodium (Na+) channels are members of a multigene family and are responsible for generation and propagation of the action potential in excitable cells. We have assembled, in a transcription-competent vector, a full-length cDNA clone encoding the rat brain type III Na+ channel. Xenopus oocytes microinjected with in vitro synthesized mRNA expressed functional rat brain Na+ channels from such 'cloned' RNA transcripts. We found that type III Na+ currents in whole cell microelectrode voltage clamp and in cell-attached patch recordings decayed much more slowly than any other reported Na+ current. In addition, we saw typical and additive effects of alpha- and beta-scorpion toxins, suggesting that the Na+ channel alpha-subunit itself contains functional and distinct toxin binding sites.Entities:
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Year: 1990 PMID： 2160038 DOI： 10.1016/0169-328x(90)90087-t
Source DB: PubMed Journal: Brain Res Mol Brain Res ISSN： 0169-328X