OBJECTIVE: The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells. MATERIALS AND METHODS: HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E(2) (PGE(2)) released into the culture medium were determined using ELISA. RESULTS: Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE(2) synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE(2) synthesis induced by mechanical stretch. CONCLUSION: We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation.
OBJECTIVE: The purpose of this study was to investigate the mechanism by which heme oxygenase-1 (HO-1) regulates inflammatory responses induced by mechanical stretch in human fibroblast-like synoviocyte (HFLS) cells. MATERIALS AND METHODS: HFLS cells were cultured in the presence of hemin and seeded into fibronectin-coated silicon chambers. The chambers were attached to a stretching apparatus which applied a uniaxial sinusoidal stretching force. The genetic expressions of cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β) and HO-1 were analyzed using real-time RT-PCR. The expression and localization of HO-1 protein were detected by immunofluorescence staining. The amounts of prostaglandin E(2) (PGE(2)) released into the culture medium were determined using ELISA. RESULTS: Mechanical stretch enhanced the expressions of COX-2 and IL-1β, and the amount of PGE(2) synthesis in HFLS cells, whereas that of HO-1 was slightly increased. In contrast, treatment with hemin enhanced HO-1 gene expression and mechanical stretch enhanced this expression in hemin-pretreated cells. In addition, hemin pretreatment suppressed PGE(2) synthesis induced by mechanical stretch. CONCLUSION: We found that constitutive HO-1 expression in hemin-pretreated HFLS cells suppressed mechanical stretch-induced inflammatory responses, suggesting that HO-1 may play a role as a regulation factor in synovial tissue inflammation.
Authors: Danielle Morse; Soeren E Pischke; Zhihong Zhou; Roger J Davis; Richard A Flavell; Torsten Loop; Sherrie L Otterbein; Leo E Otterbein; Augustine M K Choi Journal: J Biol Chem Date: 2003-07-11 Impact factor: 5.157
Authors: Niels A J Cremers; Maarten Suttorp; Marlous M Gerritsen; Ronald J Wong; Coby van Run-van Breda; Gooitzen M van Dam; Katrien M Brouwer; Anne Marie Kuijpers-Jagtman; Carine E L Carels; Ditte M S Lundvig; Frank A D T G Wagener Journal: Front Med (Lausanne) Date: 2015-12-15
Authors: Holly T Philpott; Trevor B Birmingham; Benoit Fiset; Logan A Walsh; Mitchell C Coleman; Cheryle A Séguin; C Thomas Appleton Journal: Sci Rep Date: 2022-10-17 Impact factor: 4.996