| Literature DB >> 21597466 |
Cédric Hesling1, Laurent Fattet, Guillaume Teyre, Delphine Jury, Philippe Gonzalo, Jonathan Lopez, Christophe Vanbelle, Anne-Pierre Morel, Germain Gillet, Ivan Mikaelian, Ruth Rimokh.
Abstract
TGF-β is a potent inducer of epithelial-to-mesenchymal transition (EMT), a process involved in tumour invasion. TIF1γ participates in TGF-β signalling. To understand the role of TIF1γ in TGF-β signalling and its requirement for EMT, we analysed the TGF-β1 response of human mammary epithelial cell lines. A strong EMT increase was observed in TIF1γ-silenced cells after TGF-β1 treatment, whereas Smad4 inactivation completely blocked this process. Accordingly, the functions of several TIF1γ target genes can be linked to EMT, as shown by microarray analysis. As a negative regulator of Smad4, TIF1γ could be crucial for the regulation of TGF-β signalling. Furthermore, TIF1γ binds to and represses the plasminogen activator inhibitor 1 promoter, demonstrating a direct role of TIF1γ in TGF-β-dependent gene expression. This study shows the molecular relationship between TIF1γ and Smad4 in TGF-β signalling and EMT.Entities:
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Year: 2011 PMID： 21597466 PMCID： PMC3128966 DOI： 10.1038/embor.2011.78
Source DB: PubMed Journal: EMBO Rep ISSN： 1469-221X Impact factor: 8.807