| Literature DB >> 21596559 |
Jian Shi1, Mirvat A Ebrik, Bin Yang, Rebecca J Garlock, Venkatesh Balan, Bruce E Dale, V Ramesh Pallapolu, Y Y Lee, Youngmi Kim, Nathan S Mosier, Michael R Ladisch, Mark T Holtzapple, Matthew Falls, Rocio Sierra-Ramirez, Bryon S Donohoe, Todd B Vinzant, Richard T Elander, Bonnie Hames, Steve Thomas, Ryan E Warner, Charles E Wyman.
Abstract
Accellerase 1000 cellulase, Spezyme CP cellulase, β-glucosidase, Multifect xylanase, and beta-xylosidase were evaluated for hydrolysis of pure cellulose, pure xylan, and switchgrass solids from leading pretreatments of dilute sulfuric acid, sulfur dioxide, liquid hot water, lime, soaking in aqueous ammonia, and ammonia fiber expansion. Distinctive sugar release patterns were observed from Avicel, phosphoric acid swollen cellulose (PASC), xylan, and pretreated switchgrass solids, with accumulation of significant amounts of xylooligomers during xylan hydrolysis. The strong inhibition of cellulose hydrolysis by xylooligomers could be partially attributed to the negative impact of xylooligomers on cellulase adsorption. The digestibility of pretreated switchgrass varied with pretreatment but could not be consistently correlated to xylan, lignin, or acetyl removal. Initial hydrolysis rates did correlate well with cellulase adsorption capacities for all pretreatments except lime, but more investigation is needed to relate this behavior to physical and compositional properties of pretreated switchgrass.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21596559 DOI： 10.1016/j.biortech.2011.04.003
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642