Mahsa M Amoli1, Shirin Hasani-Ranjbar2, Nahid Roohipour2, Forough A Sayahpour2, Parvin Amiri2, Parisa Zahedi2, Mahdie Mehrab-Mohseni3, Ramin Heshmat2, Bagher Larijani2, Javad Tavakkoly-Bazzaz4. 1. EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran. Electronic address: amolimm@tums.ac.ir. 2. EMRC (Endocrinology and Metabolism Research Center), Tehran University of Medical Sciences, Tehran, Iran. 3. Science and Research Branch, Islamic Azad university, Tehran, Iran. 4. Department of Medical Genetics, School of Medicine, Tehran university of Medical Sciences, Poursina St., Tehran 14176, Iran. Electronic address: tavakkolybazzazj@tums.ac.ir.
Abstract
OBJECTIVE: Functional polymorphisms within vascular endothelial growth factor (VEGF) gene have shown association with various conditions including diabetic neuropathy and retinopathy. In this study we have performed a candidate gene association study in order to examine VEGF gene polymorphism association with diabetic foot ulcer (DFU). METHODS: The study group comprised of type 2 diabetes patients with (N=247) and without (N=241) DFU. Healthy control subjects (N=98) were also recruited from the same area. The ARMS-PCR technique was applied for genotyping of VEGF gene SNPs at positions -7*C/T and -2578*C/A. RESULTS: The frequency of genotype AA was significantly decreased in patients with DFU compared with diabetic subjects without DFU (AA vs CA+CC, p=0.003, OR=0.44, CI=0.24-0.80). Also there was a significant decrease in frequency of A allele in patients with DFU compared to the controls (p=0.02, OR=0.68, CI=0.48-0.96). CONCLUSION: It seems that lower frequency of A allele in patients with DFU is conferring a protective effect which might be as a result of increased angiogenesis in patients carrying this allele.
OBJECTIVE: Functional polymorphisms within vascular endothelial growth factor (VEGF) gene have shown association with various conditions including diabetic neuropathy and retinopathy. In this study we have performed a candidate gene association study in order to examine VEGF gene polymorphism association with diabetic foot ulcer (DFU). METHODS: The study group comprised of type 2 diabetespatients with (N=247) and without (N=241) DFU. Healthy control subjects (N=98) were also recruited from the same area. The ARMS-PCR technique was applied for genotyping of VEGF gene SNPs at positions -7*C/T and -2578*C/A. RESULTS: The frequency of genotype AA was significantly decreased in patients with DFU compared with diabetic subjects without DFU (AA vs CA+CC, p=0.003, OR=0.44, CI=0.24-0.80). Also there was a significant decrease in frequency of A allele in patients with DFU compared to the controls (p=0.02, OR=0.68, CI=0.48-0.96). CONCLUSION: It seems that lower frequency of A allele in patients with DFU is conferring a protective effect which might be as a result of increased angiogenesis in patients carrying this allele.
Authors: Imadeldin Elfaki; Rashid Mir; Faisel M Abu Duhier; Maeidh A Alotaibi; Adel Ibrahim Alalawy; Jameel Barnawi; Abdullatif Taha Babakr; Mohammad Muzaffar Mir; Faris Altayeb; Hyder Mirghani; Ehab A M Frah Journal: Curr Issues Mol Biol Date: 2021-11-02 Impact factor: 2.976