BACKGROUND: The objective of this study was to determine if neonatal cystitis alters colonic sensitivity later in life and to investigate the role of peripheral mechanisms. METHODS: Neonatal rats received intravesical zymosan, normal saline, or anesthesia only for three consecutive days [(postnatal (PN) days 14-16)]. The estrous cycle phase was determined prior to recording the visceromotor response (VMR) to colorectal distension (CRD) in adult rats. Eosinophils and mast cells were examined from colon and bladder tissues. CRD- or urinary bladder distension (UBD)-sensitive pelvic nerve afferents (PNAs) were identified and their responses to distension were examined. The relative expression of N-methyl-d-aspartic acid (NMDA)-NR1 subunit in the lumbo-sacral (L6-S1) spinal cord was examined using Western blot. KEY RESULTS: The VMR to CRD (≥10mmHg) in the neonatal zymosan group was significantly higher than control in both the diestrus, estrus phase and in all phases combined. There was no difference in the total number of eosinophils, mast cells or number of degranulated mast cells between groups. The spontaneous firing of UBD, but not CRD-sensitive PNAs from the zymosan-treated rats was significantly higher than the saline-treated control. However, the mechanosensitive properties of PNAs to CRD or UBD were no different between groups (P>0.05). The expression of spinal NR1 subunit was significantly higher in zymosan-treated rats compared with saline-treated rats (P<0.05). CONCLUSIONS & INFERENCES: Neonatal cystitis results in colonic hypersensitivity in adult rats without changing tissue histology or the mechanosensitive properties of CRD-sensitive PNAs. Neonatal cystitis does result in overexpression of spinal NR1 subunit in adult rats.
BACKGROUND: The objective of this study was to determine if neonatal cystitis alters colonic sensitivity later in life and to investigate the role of peripheral mechanisms. METHODS: Neonatal rats received intravesical zymosan, normal saline, or anesthesia only for three consecutive days [(postnatal (PN) days 14-16)]. The estrous cycle phase was determined prior to recording the visceromotor response (VMR) to colorectal distension (CRD) in adult rats. Eosinophils and mast cells were examined from colon and bladder tissues. CRD- or urinary bladder distension (UBD)-sensitive pelvic nerve afferents (PNAs) were identified and their responses to distension were examined. The relative expression of N-methyl-d-aspartic acid (NMDA)-NR1 subunit in the lumbo-sacral (L6-S1) spinal cord was examined using Western blot. KEY RESULTS: The VMR to CRD (≥10mmHg) in the neonatal zymosan group was significantly higher than control in both the diestrus, estrus phase and in all phases combined. There was no difference in the total number of eosinophils, mast cells or number of degranulated mast cells between groups. The spontaneous firing of UBD, but not CRD-sensitive PNAs from the zymosan-treated rats was significantly higher than the saline-treated control. However, the mechanosensitive properties of PNAs to CRD or UBD were no different between groups (P>0.05). The expression of spinal NR1 subunit was significantly higher in zymosan-treated rats compared with saline-treated rats (P<0.05). CONCLUSIONS & INFERENCES: Neonatal cystitis results in colonic hypersensitivity in adult rats without changing tissue histology or the mechanosensitive properties of CRD-sensitive PNAs. Neonatal cystitis does result in overexpression of spinal NR1 subunit in adult rats.
Authors: Ruomei Liang; Elena E Ustinova; Radhika Patnam; Matthew O Fraser; Dmitriy W Gutkin; Michael A Pezzone Journal: Neurourol Urodyn Date: 2007 Impact factor: 2.696
Authors: Denesh K Chitkara; Miranda A L van Tilburg; Nannette Blois-Martin; William E Whitehead Journal: Am J Gastroenterol Date: 2008-01-02 Impact factor: 10.864
Authors: Julie A Christianson; Ruomei Liang; Elena E Ustinova; Brian M Davis; Matthew O Fraser; Michael A Pezzone Journal: Pain Date: 2006-10-27 Impact factor: 7.926
Authors: Anna P Malykhina; Jean-Jacques Wyndaele; Karl-Erik Andersson; Stefan De Wachter; Roger R Dmochowski Journal: Neurourol Urodyn Date: 2012-02-29 Impact factor: 2.696
Authors: B Banerjee; B K Medda; J Zhang; V Tuchscherer; R Babygirija; P Kannampalli; J N Sengupta; R Shaker Journal: Neurogastroenterol Motil Date: 2016-06-08 Impact factor: 3.598
Authors: Maia Terashvili; Bhavana Talluri; Watchareepohn Palangmonthip; Kenneth A Iczkowski; Patrick Sanvanson; Bidyut K Medda; Banani Banerjee; Christopher W Cunningham; Jyoti N Sengupta Journal: Neuropharmacology Date: 2021-07-10 Impact factor: 5.273