Literature DB >> 2158610

Solubilization and partial purification of cholinephosphotransferase in hamster tissues.

K M O1, P C Choy.   

Abstract

CDPcholine:1,2-diacylglycerol cholinephosphotransferase (EC 2.7.8.2) is located on the cytoplasmic side of the endoplasmic reticulum, and catalyzes the final step in the synthesis of phosphatidylcholine via the CDPcholine pathway. The enzyme was solubilized from hamster liver microsomes by 3% Triton QS-15, and partially purified by DEAE-Sepharose chromatography and Sepharose 6B chromatography. The microsomal and partially purified enzymes displayed similar pH profile, and both showed absolute requirement for Mg++ or other divalent cations. The Km values of CDPcholine were similar between microsomal and partially purified enzyme, whereas the Km value for diacylglycerol was substantially lowered when the enzyme was partially purified. Hamster heart cholinephosphotransferase was not solubilized by Triton QS-15.

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Year:  1990        PMID： 2158610     DOI： 10.1007/BF02562217

Source DB:  PubMed          Journal:  Lipids        ISSN： 0024-4201            Impact factor:   1.880


  13 in total

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Authors:  S Parthasarathy; W J Baumann
Journal:  Biochem Biophys Res Commun       Date:  1979-11-28       Impact factor: 3.575

2.  Acyl chain length dependency of diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase. Effect of different saturated fatty acids at the C-1 or C-2 position of diacylglycerol on solubilized rat liver microsomal enzymes.

Authors:  K Morimoto; H Kanoh
Journal:  J Biol Chem       Date:  1978-07-25       Impact factor: 5.157

3.  Differential utilization of 1-palmitoyl and 1-stearoyl homologues of various unsaturated 1,2-diacyl-sn-glycerols for phosphatidylcholine and phosphatidylethanolamine synthesis in rat liver microsomes.

Authors:  B J Holub
Journal:  J Biol Chem       Date:  1978-02-10       Impact factor: 5.157

4.  Asymmetry of phospholipid biosynthesis.

Authors:  D E Vance; P C Choy; S B Farren; P H Lim; W J Schneider
Journal:  Nature       Date:  1977-11-17       Impact factor: 49.962

5.  Solubilization and reconstitution of cholinephosphotransferase from sarcoplasmic reticulum: stabilization of solubilized enzyme by diacylglycerol and glycerol.

Authors:  R Cornell; D H MacLennan
Journal:  Biochim Biophys Acta       Date:  1985-11-21

Review 6.  Lipid regulation of cell membrane structure and function.

Authors:  P L Yeagle
Journal:  FASEB J       Date:  1989-05       Impact factor: 5.191

7.  The structural specificity of lecithin for activation of purified D-beta-hydroxybutyrate apodehydrogenase.

Authors:  Y A Isaacson; P W Deroo; A F Rosenthal; R Bittman; J O McIntyre; H G Bock; P Gazzotti; S Fleischer
Journal:  J Biol Chem       Date:  1979-01-10       Impact factor: 5.157

8.  Hamster liver cholinephosphotransferase and ethanolaminephosphotransferase are separate enzymes.

Authors:  K M O; Y L Siow; P C Choy
Journal:  Biochem Cell Biol       Date:  1989-10       Impact factor: 3.626

Review 9.  Regulation of phosphatidylcholine metabolism in mammalian hearts.

Authors:  G M Hatch; K O; P C Choy
Journal:  Biochem Cell Biol       Date:  1989 Feb-Mar       Impact factor: 3.626

10.  The catabolism of plasmenylcholine in the guinea pig heart.

Authors:  G Arthur; L Page; T Mock; P C Choy
Journal:  Biochem J       Date:  1986-06-01       Impact factor: 3.857
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  10 in total

1.  Control of membrane phosphatidylcholine biosynthesis by diacylglycerol levels in neuronal cells undergoing neurite outgrowth.

Authors:  W Araki; R J Wurtman
Journal:  Proc Natl Acad Sci U S A       Date:  1997-10-28       Impact factor: 11.205

2.  The modulation of choline phosphoglyceride metabolism in human colon cancer.

Authors:  D A Dueck; M Chan; K Tran; J T Wong; F T Jay; C Littman; R Stimpson; P C Choy
Journal:  Mol Cell Biochem       Date:  1996-09-20       Impact factor: 3.396

3.  Effects of fasting on phosphatidylcholine biosynthesis in hamster liver: regulation of cholinephosphotransferase activity by endogenous argininosuccinate.

Authors:  K M O; P C Choy
Journal:  Biochem J       Date:  1993-02-01       Impact factor: 3.857

4.  Biphasic modulation of choline uptake and phosphatidylcholine biosynthesis by vasopressin in rat cardiac myocytes.

Authors:  A S Man; E Lee; P C Choy
Journal:  Lipids       Date:  1994-01       Impact factor: 1.880

5.  Plasmenylcholine (1-O-alk-1'-enyl-2-acyl-sn-glycero-3-phosphocholine) biosynthesis in guinea-pig heart and liver: cholinephosphotransferase is a bifunctional enzyme for the synthesis of phosphatidylcholine and plasmenylcholine.

Authors:  Y F Xu; K O; P C Choy
Journal:  Biochem J       Date:  1994-07-01       Impact factor: 3.857

6.  Phosphatidylcholine metabolism in ischemic and hypoxic hearts.

Authors:  P C Choy; M Chan; G Hatch; R Y Man
Journal:  Mol Cell Biochem       Date:  1992-10-21       Impact factor: 3.396

7.  The effect of methyl-lidocaine on the biosynthesis of phospholipids de novo in the isolated hamster heart.

Authors:  P G Tardi; R Y Man; P C Choy
Journal:  Biochem J       Date:  1992-07-01       Impact factor: 3.857

8.  CDPcholine:1,2-diacylglycerol cholinephosphotransferase from rat liver microsomes. I. Solubilization and characterization of the partially purified enzyme and the possible existence of an endogenous inhibitor.

Authors:  K Ishidate; R Matsuo; Y Nakazawa
Journal:  Lipids       Date:  1993-02       Impact factor: 1.880

Review 9.  Oral administration of circulating precursors for membrane phosphatides can promote the synthesis of new brain synapses.

Authors:  Mehmet Cansev; Richard J Wurtman; Toshimasa Sakamoto; Ismail H Ulus
Journal:  Alzheimers Dement       Date:  2007-12-21       Impact factor: 21.566

10.  PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1).

Authors:  Marcia M Wright; Christopher R McMaster
Journal:  Lipids       Date:  2002-07       Impact factor: 1.880
  10 in total

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