Literature DB >> 21585869

Group-specific primers for DNA-based detection of springtails (Hexapoda: Collembola) within predator gut contents.

A K Kuusk1, N Agustí.   

Abstract

Group-specific, degenerate polymerase chain reaction primers for DNA-based detection of springtails (Hexapoda: Collembola) within predator gut contents have been developed for the first time. Primers were designed from 18S rDNA and amplified fragments of 272 bp and 177 bp from 17 springtail species collected in agricultural habitats. Specificity tests against 41 nontarget species revealed no cross-reactivity. Group-specific polymerase chain reaction is advantageous when working in species-rich habitats and these primers could facilitate studies of trophic links between springtails and generalist arthropod predators worldwide.
© 2007 The Authors.

Year:  2008        PMID： 21585869     DOI： 10.1111/j.1471-8286.2007.02046.x

Source DB:  PubMed          Journal:  Mol Ecol Resour        ISSN： 1755-098X            Impact factor:   7.090


  6 in total

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Authors:  Britta Frei; Yasemin Guenay; David A Bohan; Michael Traugott; Corinna Wallinger
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2.  Advances in multiplex PCR: balancing primer efficiencies and improving detection success.

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Authors:  Daniela Sint; Bettina Niederklapfer; Ruediger Kaufmann; Michael Traugott
Journal:  PLoS One       Date:  2014-12-19       Impact factor: 3.240

4.  Diagnostic PCR assays to unravel food web interactions in cereal crops with focus on biological control of aphids.

Authors:  Karin Staudacher; Mattias Jonsson; Michael Traugott
Journal:  J Pest Sci (2004)       Date:  2015-08-11       Impact factor: 5.918

5.  Spatial subsidies in spider diets vary with shoreline structure: Complementary evidence from molecular diet analysis and stable isotopes.

Authors:  Peter A Hambäck; Elisabeth Weingartner; Love Dalén; Helena Wirta; Tomas Roslin
Journal:  Ecol Evol       Date:  2016-10-26       Impact factor: 2.912

6.  When to use next generation sequencing or diagnostic PCR in diet analyses.

Authors:  Oskar Rennstam Rubbmark; Daniela Sint; Sandra Cupic; Michael Traugott
Journal:  Mol Ecol Resour       Date:  2019-02-04       Impact factor: 7.090
  6 in total

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