| Literature DB >> 21585866 |
M McHale1, D Broderick, J R Ovenden, J M Lanyon.
Abstract
Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.Entities:
Year: 2008 PMID： 21585866 DOI： 10.1111/j.1471-8286.2007.02041.x
Source DB: PubMed Journal: Mol Ecol Resour ISSN： 1755-098X Impact factor: 7.090