| Literature DB >> 21585761 |
Nicholas G Crawford1, Jaime Zaldívar-Rae, Cris Hagen, Amanda Schable, Erica Bree Rosenblum, Jeff A Graves, Tod W Reeder, Michael G Ritchie, Travis C Glenn.
Abstract
We describe polymerase chain reaction primers and amplification conditions for 13 microsatellite DNA loci isolated from two bisexual species of whiptail lizards Aspidoscelis costata huico and Aspidoscelis inornata. Primers were tested on either 16 or 48 individuals of A. c. huico and/or 26 individuals of A. inornata. Ten of the 13 primers were also tested against a panel of 31 additional whiptail taxa. We detected three to nine alleles per locus in A. c. huico and four to 19 alleles per locus in A. inornata, with observed heterozygosity ranging from 0.60 to 0.87 and from 0.15 to 1.00, respectively. These primers will be an important resource for surveys of genetic variation in these lizards.Entities:
Year: 2008 PMID: 21585761 DOI: 10.1111/j.1471-8286.2007.01930.x
Source DB: PubMed Journal: Mol Ecol Resour ISSN: 1755-098X Impact factor: 7.090