Literature DB >> 2158336

Subtraction hybridization cDNA libraries from colon carcinoma and hepatic cancer.

C W Schweinfest1, K W Henderson, J R Gu, S D Kottaridis, S Besbeas, E Panotopoulou, T S Papas.   

Abstract

cDNA clones of differentially expressed mRNAs in a colon carcinoma and a hepatocellular carcinoma have been isolated by subtractive cDNA cloning. The subtracted material is at least 90 X enriched for differentially expressed sequences and can be used for construction of subtractive cDNA libraries and polymerase chain reaction (PCR) amplification to generate differential probes. Commercially available lambda ZAP II is used for construction of primary libraries since single-stranded phage bearing the cloned cDNA can be excised in vivo and because lambda libraries are convenient for subsequent screening and manipulations. Rare mRNAs (less than 0.01% abundance), which are differentially expressed, can be isolated utilizing this procedure.

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Year:  1990        PMID： 2158336     DOI： 10.1016/0735-0651(90)90042-e

Source DB:  PubMed          Journal:  Genet Anal Tech Appl        ISSN： 1050-3862


  14 in total

1.  Brachyury downstream notochord differentiation in the ascidian embryo.

Authors:  H Takahashi; K Hotta; A Erives; A Di Gregorio; R W Zeller; M Levine; N Satoh
Journal:  Genes Dev       Date:  1999-06-15       Impact factor: 11.361

2.  Molecular markers for cell types of the inner ear and candidate genes for hearing disorders.

Authors:  S Heller; C A Sheane; Z Javed; A J Hudspeth
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-15       Impact factor: 11.205

3.  A method for generating subtractive cDNA libraries retaining clones containing repetitive elements.

Authors:  C T Lin; D R Sargan
Journal:  Nucleic Acids Res       Date:  1997-11-01       Impact factor: 16.971

4.  Expression of a gene encoding a tRNA synthetase-like protein is enhanced in tumorigenic human myeloid leukemia cells and is cell cycle stage- and differentiation-dependent.

Authors:  S Sen; H Zhou; T Ripmaster; W N Hittelman; P Schimmel; R A White
Journal:  Proc Natl Acad Sci U S A       Date:  1997-06-10       Impact factor: 11.205

5.  An overexpressed gene transcript in senescent and quiescent human fibroblasts encoding a novel protein in the epidermal growth factor-like repeat family stimulates DNA synthesis.

Authors:  B Lecka-Czernik; C K Lumpkin; S Goldstein
Journal:  Mol Cell Biol       Date:  1995-01       Impact factor: 4.272

6.  A novel ligation mediated-PCR based strategy for construction of subtraction libraries from limiting amounts of mRNA.

Authors:  S Ghosh
Journal:  Nucleic Acids Res       Date:  1996-02-15       Impact factor: 16.971

7.  Isolation of cDNAs encoding guanine nucleotide-binding protein beta-subunit homologues from maize (ZGB1) and Arabidopsis (AGB1).

Authors:  C A Weiss; C W Garnaat; K Mukai; Y Hu; H Ma
Journal:  Proc Natl Acad Sci U S A       Date:  1994-09-27       Impact factor: 11.205

8.  An improved method for generating subtracted cDNA libraries using phage lambda vectors.

Authors:  H Hesse; W B Frommer; L Willmitzer
Journal:  Nucleic Acids Res       Date:  1995-08-25       Impact factor: 16.971

9.  A novel blue light- and abscisic acid-inducible gene of Arabidopsis thaliana encoding an intrinsic membrane protein.

Authors:  R Kaldenhoff; A Kölling; G Richter
Journal:  Plant Mol Biol       Date:  1993-12       Impact factor: 4.076

10.  Identification of a colon mucosa gene that is down-regulated in colon adenomas and adenocarcinomas.

Authors:  C W Schweinfest; K W Henderson; S Suster; N Kondoh; T S Papas
Journal:  Proc Natl Acad Sci U S A       Date:  1993-05-01       Impact factor: 11.205
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