| Literature DB >> 2157731 |
D L Krah1, T L Schofield, P J Provost.
Abstract
The infectivity titers of varicella-zoster virus (VZV) are routinely estimated by plaque production in cell culture. In this report, we show that plaque counts for VZV (strain Oka/Merck), in MRC-5 cell cultures, are significantly enhanced (54% average enhancement) by the use of an agarose overlay medium, as compared to a fluid overlay medium. Evidence also is presented that less variability (P less than 0.05) in plaque counts occurs with the use of an agarose overlay medium.Entities:
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Year: 1990 PMID： 2157731 DOI： 10.1016/0166-0934(90)90100-t
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014