| Literature DB >> 21576001 |
Weina Jin1, Qinghua Li, Yani Lin, Ying Lu, Huawen Li, Lihong Wang, Ronghua Hu, Li Ma, Jianxiang Wang, Tianxiang Pang.
Abstract
The present study was undertaken to estimate the therapeutic benefit to down-regulate the Na(+)/H(+) exchanger 1 (NHE1) for reversing chemoresistance of BCR-ABL-positive leukemia patient cells and cell lines. As a result, after treatment with specific NHE1 inhibitor Cariporide or high K(+) buffer to decrease intracellular pH (pH(i)), cells from relapsed patients exhibited decreased Pgp level, enhanced Rhodamine123 and drug accumulation, decreased colony-forming ability and the modulations of mitogen-activated protein kinases (MAPKs) activities. Furthermore, we used BCR-ABL-positive cell line K562 and its resistant counterparts K562/DOX and K562/G01 cell lines for further study. Together, these findings suggest that Pgp may be associated with the reversal of drug resistance in BCR-ABL-positive leukemia patients and cell lines by the inhibition of NHE1 though MAPK pathways.Entities:
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Year: 2011 PMID： 21576001 DOI： 10.1016/j.canlet.2011.04.016
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679