Literature DB >> 21571752

Improved preservation of fine structure of deep-sea microorganisms by freeze-substitution after glutaraldehyde fixation.

Masashi Yamaguchi1, Yuichi Namiki, Hitoshi Okada, Katsuyuki Uematsu, Akihiro Tame, Tadashi Maruyama, Yoshimichi Kozuka.   

Abstract

A method was proposed for improving preservation of ultrastructures of deep-sea microorganisms by using rapid-freeze freeze-substitution after glutaraldehyde fixation. This method produced clear high-resolution images of cells appearing in their natural state, close to the quality of images obtained by rapidly freezing freeze-substituted specimens of living cells. The method may be useful for observing any microorganism when rapid freezing of living samples is difficult and only glutaraldehyde fixation can be carried out.

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Year:  2011        PMID： 21571752     DOI： 10.1093/jmicro/dfr032

Source DB:  PubMed          Journal:  J Electron Microsc (Tokyo)        ISSN： 0022-0744


  5 in total

1.  Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in chronic myelogenous leukemia cells.

Authors:  Takuya Hirao; Masashi Yamaguchi; Megumi Kikuya; Hiroji Chibana; Kousei Ito; Shigeki Aoki
Journal:  Cancer Sci       Date:  2017-12-07       Impact factor: 6.716

2.  KLF4 is required for suppression of histamine synthesis by polyamines during bone marrow-derived mast cell differentiation.

Authors:  Kazuhiro Nishimura; Moemi Okamoto; Rina Shibue; Toshio Mizuta; Toru Shibayama; Tetsuhiko Yoshino; Teruki Murakami; Masashi Yamaguchi; Satoshi Tanaka; Toshihiko Toida; Kazuei Igarashi
Journal:  PLoS One       Date:  2020-02-26       Impact factor: 3.240

3.  Multispecies Populations of Methanotrophic Methyloprofundus and Cultivation of a Likely Dominant Species from the Iheya North Deep-Sea Hydrothermal Field.

Authors:  Hisako Hirayama; Yoshihiro Takaki; Mariko Abe; Hiroyuki Imachi; Tetsuro Ikuta; Junichi Miyazaki; Eiji Tasumi; Katsuyuki Uematsu; Akihiro Tame; Miwako Tsuda; Keiko Tanaka; Yohei Matsui; Hiromi Kayama Watanabe; Hiroyuki Yamamoto; Ken Takai
Journal:  Appl Environ Microbiol       Date:  2021-11-17       Impact factor: 5.005

4.  Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells.

Authors:  Shigeki Aoki; Michie Morita; Takuya Hirao; Masashi Yamaguchi; Reika Shiratori; Megumi Kikuya; Hiroji Chibana; Kousei Ito
Journal:  Oncotarget       Date:  2017-10-09

5.  Glycolytic suppression dramatically changes the intracellular metabolic profile of multiple cancer cell lines in a mitochondrial metabolism-dependent manner.

Authors:  Reika Shiratori; Kenta Furuichi; Masashi Yamaguchi; Natsumi Miyazaki; Haruna Aoki; Hiroji Chibana; Kousei Ito; Shigeki Aoki
Journal:  Sci Rep       Date:  2019-12-10       Impact factor: 4.379
  5 in total

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