PURPOSE: To determine the levels of 23 immune mediators in paired aqueous humor (AqH) and serum samples from patients with birdshot chorioretinopathy (BSCR). DESIGN: Single-centre case-control study. METHODS: A multiplex immunoassay was used to determine the levels of 23 immune mediators (T-cell, proinflammatory, and vascular-active mediators) in paired AqH and serum of 16 BSCR patients. The AqH of 11 age-related cataract controls served as controls. RESULTS: AqH levels of the T-cell mediators interleukin (IL)-2 (P=.044) and IL-17 (P=.039) and proinflammatory mediators IL-1β (P=.032), IL-6 (P=.034), and tumor necrosis factor α (P=.041) were elevated compared with that of age-related cataract controls. The elevated intraocular levels of IL-1β, IL-17, and tumor necrosis factor α in BSCR samples were higher than their concurrent serum levels. A significant positive correlation of intraocular mediators was noted between IL-17 and both IL-2 (r=0.744; P<.0001) and IL-23 (r=0.921; P<.0001) and between IL-2 and IL-23 (r=0.776; P<.0001). AqH levels of vascular-active mediators were not distinct between the groups. CONCLUSIONS: BSCR patients have elevated intraocular levels of proinflammatory and T cell-associated cytokines. Our results suggest the novel pathogenic concept that BSCR is an autoimmune inflammatory disease restricted to the eye and associated with elevated IL-17.
PURPOSE: To determine the levels of 23 immune mediators in paired aqueous humor (AqH) and serum samples from patients with birdshot chorioretinopathy (BSCR). DESIGN: Single-centre case-control study. METHODS: A multiplex immunoassay was used to determine the levels of 23 immune mediators (T-cell, proinflammatory, and vascular-active mediators) in paired AqH and serum of 16 BSCR patients. The AqH of 11 age-related cataract controls served as controls. RESULTS: AqH levels of the T-cell mediators interleukin (IL)-2 (P=.044) and IL-17 (P=.039) and proinflammatory mediators IL-1β (P=.032), IL-6 (P=.034), and tumor necrosis factor α (P=.041) were elevated compared with that of age-related cataract controls. The elevated intraocular levels of IL-1β, IL-17, and tumor necrosis factor α in BSCR samples were higher than their concurrent serum levels. A significant positive correlation of intraocular mediators was noted between IL-17 and both IL-2 (r=0.744; P<.0001) and IL-23 (r=0.921; P<.0001) and between IL-2 and IL-23 (r=0.776; P<.0001). AqH levels of vascular-active mediators were not distinct between the groups. CONCLUSIONS: BSCR patients have elevated intraocular levels of proinflammatory and T cell-associated cytokines. Our results suggest the novel pathogenic concept that BSCR is an autoimmune inflammatory disease restricted to the eye and associated with elevated IL-17.
Authors: Jonas J W Kuiper; Jessica Van Setten; Stephan Ripke; Ruben Van 'T Slot; Flip Mulder; Tom Missotten; G Seerp Baarsma; Laurent C Francioli; Sara L Pulit; Carolien G F De Kovel; Ninette Ten Dam-Van Loon; Anneke I Den Hollander; Paulien Huis in het Veld; Carel B Hoyng; Miguel Cordero-Coma; Javier Martín; Victor Llorenç; Bharti Arya; Dhanes Thomas; Steven C Bakker; Roel A Ophoff; Aniki Rothova; Paul I W De Bakker; Tuna Mutis; Bobby P C Koeleman Journal: Hum Mol Genet Date: 2014-06-22 Impact factor: 6.150
Authors: Alejandro Sanz-Bravo; Adrian Martín-Esteban; Jonas J W Kuiper; Marina García-Peydró; Eilon Barnea; Arie Admon; José A López de Castro Journal: Mol Cell Proteomics Date: 2018-05-16 Impact factor: 5.911
Authors: Arpita S Bharadwaj; Binoy Appukuttan; Phillip A Wilmarth; Yuzhen Pan; Andrew J Stempel; Timothy J Chipps; Eric E Benedetti; David O Zamora; Dongseok Choi; Larry L David; Justine R Smith Journal: Prog Retin Eye Res Date: 2012-09-11 Impact factor: 21.198