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Literature DB >> 2157

Initial rates of pyruvate transport in mitochondria determined by an "inhibitor-stop" technique.

Abstract

An "inhibitor-stop" technique has been developed for measuring initial rates of pyruvate transport into mitochondria. The technique uses alpha-cyano-3-hydroxycinnamate as the inhibitor and separates the mitochondria from the radioactive medium by Millipore filtration. Observed rates depend on availability of hydroxyl and other exchangeable anions within the mitochondrial matrix.

Entities: Chemical

Mesh：

Substances：

Year: 1975 PMID： 2157 PMCID： PMC1165770 DOI： 10.1042/bj1500553

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

11 in total

1. The transport of pyruvate in rat liver mitochondria.

Authors: S Papa; A Francavilla; G Paradies; B Meduri
Journal: FEBS Lett Date: 1971-01-30 Impact factor: 4.124

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. Evidence for the role of a specific monocarboxylate transporter in the control of pyruvate oxidation by rat liver mitochondria.

Authors: J Mowbray
Journal: FEBS Lett Date: 1974-08-30 Impact factor: 4.124

8. The intracellular localization of enzymes in white-adipose-tissue fat-cells and permeability properties of fat-cell mitochondria. Transfer of acetyl units and reducing power between mitochondria and cytoplasm.

Authors: B R Martin; R M Denton
Journal: Biochem J Date: 1970-05 Impact factor: 3.857

9. Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by alpha-cyano-4-hydroxycinnamate.

Authors: A P Halestrap; R M Denton
Journal: Biochem J Date: 1974-02 Impact factor: 3.857

10. Quantitative characteristics of glutamate transport in rat liver mitochondria.

Authors: N M Bradford; J D McGivan
Journal: Biochem J Date: 1973-08 Impact factor: 3.857

3 in total

1. Pyruvate and ketone-body transport across the mitochondrial membrane. Exchange properties, pH-dependence and mechanism of the carrier.

Authors: A P Halestrap
Journal: Biochem J Date: 1978-06-15 Impact factor: 3.857

Review 2. Mitochondrial pyruvate transport: a historical perspective and future research directions.

Authors: Kyle S McCommis; Brian N Finck
Journal: Biochem J Date: 2015-03-15 Impact factor: 3.857

3. A Method for Multiplexed Measurement of Mitochondrial Pyruvate Carrier Activity.

Authors: Lawrence R Gray; Adam J Rauckhorst; Eric B Taylor
Journal: J Biol Chem Date: 2016-01-28 Impact factor: 5.157