Comutagenesis-II. Some factors influencing the in vitro metabolism of 2-amino-3-methylpyridine.

Factors affecting the metabolism of 2-amino-3-methylpyridine (2A3MP) in vitro have been studied and the conditions which allow maximal metabolism established. Ring nuclear and methyl hydroxylation, and 1-N-oxidation of 2A3MP were linear with respect to arochlor 1254 induced rat S9 supernatant (10,000 g fraction) up to 4.86 mg per ml. The results showed that 20 min incubation time was adequate to observe metabolites formed from 2A3MP. The rate of metabolite production increased with increase in substrate concentration up to 2 μmol per incubate. Using the data obtained the apparent K(m) and V(max) values were calculated using Hanes-Wolf and Lineweaver-Burk plot. No N-hydroxylation of the exo-amino group was observed.