| Literature DB >> 21566084 |
Gustavo A Miranda-Carboni1, Miriam Guemes, Shannon Bailey, Edgar Anaya, Mirko Corselli, Bruno Peault, Susan A Krum.
Abstract
Estrogens regulate osteoblast differentiation and mineralization. We identified GATA4 as a transcription factor expressed in osteoblasts and directly regulated by 17β-estradiol in this cell type but not in breast cancer cells, another estrogen-responsive tissue. Chromatin immunoprecipitation sequencing (chromatin immunoprecipitation sequencing) reveals that estrogen receptor α (ERα) binds to chromatin near GATA4 at five different enhancers. GATA4 and ERα are both recruited to ERα binding sites near genes that are specifically expressed in osteoblasts and control osteoblast differentiation. Maximal binding of GATA4 precedes ERα binding, and GATA4 is necessary for histone 3 lysine 4 dimethylation at ERα binding sites, suggesting that GATA4 is a pioneer factor for ERα. As such, knockdown of GATA4 reduced recruitment of ERα to DNA. Our study illustrates that GATA4 is a pioneer factor for ERα recruitment to osteoblast-specific enhancers.Entities:
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Year: 2011 PMID： 21566084 PMCID： PMC3125091 DOI： 10.1210/me.2010-0463
Source DB: PubMed Journal: Mol Endocrinol ISSN： 0888-8809