Literature DB >> 21565009

High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies.

B L Smith1, C-P Lu, J R Alvarado Bremer.   

Abstract

High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.
© 2009 Blackwell Publishing Ltd.

Entities:  

Year:  2009        PMID： 21565009     DOI： 10.1111/j.1755-0998.2009.02726.x

Source DB:  PubMed          Journal:  Mol Ecol Resour        ISSN： 1755-098X            Impact factor:   7.090


  16 in total

Review 1.  Rapid and cost-effective screening of newly identified microsatellite loci by high-resolution melting analysis.

Authors:  Wolfgang Arthofer; Florian M Steiner; Birgit C Schlick-Steiner
Journal:  Mol Genet Genomics       Date:  2011-08-17       Impact factor: 3.291

2.  Evolution of ASPM coding variation in apes and associations with brain structure in chimpanzees.

Authors:  Sheel V Singh; Nicky Staes; Elaine E Guevara; Steven J Schapiro; John J Ely; William D Hopkins; Chet C Sherwood; Brenda J Bradley
Journal:  Genes Brain Behav       Date:  2019-06-11       Impact factor: 3.449

3.  Primate genotyping via high resolution melt analysis: rapid and reliable identification of color vision status in wild lemurs.

Authors:  Rachel L Jacobs; Amanda N Spriggs; Tammie S MacFie; Andrea L Baden; Mitchell T Irwin; Patricia C Wright; Edward E Louis; Richard R Lawler; Nicholas I Mundy; Brenda J Bradley
Journal:  Primates       Date:  2016-06-06       Impact factor: 2.163

4.  Serotonin Receptor 1A Variation Is Associated with Anxiety and Agonistic Behavior in Chimpanzees.

Authors:  Nicky Staes; Chet C Sherwood; Hani Freeman; Sarah F Brosnan; Steven J Schapiro; William D Hopkins; Brenda J Bradley
Journal:  Mol Biol Evol       Date:  2019-07-01       Impact factor: 16.240

5.  Identification of a polymorphism within the Rosa multiflora muRdr1A gene linked to resistance to multiple races of Diplocarpon rosae W. in tetraploid garden roses (Rosa × hybrida).

Authors:  Cindy Rouet; Elizabeth A Lee; Travis Banks; Joseph O'Neill; Rachael LeBlanc; Daryl J Somers
Journal:  Theor Appl Genet       Date:  2019-09-28       Impact factor: 5.699

6.  Application of high-resolution DNA melting for genotyping in lepidopteran non-model species: Ostrinia furnacalis (Crambidae).

Authors:  FengBo Li; BaoLong Niu; YongPing Huang; ZhiQi Meng
Journal:  PLoS One       Date:  2012-01-11       Impact factor: 3.240

7.  Multilocus Bayesian Estimates of Intra-Oceanic Genetic Differentiation, Connectivity, and Admixture in Atlantic Swordfish (Xiphias gladius L.).

Authors:  Brad L Smith; Ching-Ping Lu; Blanca García-Cortés; Jordi Viñas; Shean-Ya Yeh; Jaime R Alvarado Bremer
Journal:  PLoS One       Date:  2015-06-09       Impact factor: 3.240

8.  A toolkit for bulk PCR-based marker design from next-generation sequence data: application for development of a framework linkage map in bulb onion (Allium cepa L.).

Authors:  Samantha Baldwin; Roopashree Revanna; Susan Thomson; Meeghan Pither-Joyce; Kathryn Wright; Ross Crowhurst; Mark Fiers; Leshi Chen; Richard Macknight; John A McCallum
Journal:  BMC Genomics       Date:  2012-11-19       Impact factor: 3.969

9.  Spatial, temporal, and habitat-related variation in abundance of pelagic fishes in the Gulf of Mexico: potential implications of the deepwater horizon oil spill.

Authors:  Jay R Rooker; Larissa L Kitchens; Michael A Dance; R J David Wells; Brett Falterman; Maëlle Cornic
Journal:  PLoS One       Date:  2013-10-10       Impact factor: 3.240

10.  Identification of pummelo cultivars by using a panel of 25 selected SNPs and 12 DNA segments.

Authors:  Bo Wu; Guang-yan Zhong; Jian-qiang Yue; Run-ting Yang; Chong Li; Yue-jia Li; Yun Zhong; Xuan Wang; Bo Jiang; Ji-wu Zeng; Li Zhang; Shu-tang Yan; Xue-jun Bei; Dong-guo Zhou
Journal:  PLoS One       Date:  2014-04-14       Impact factor: 3.240
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