Taro Nonaka1, Koichi Takakuwa, Kenichi Tanaka. 1. Department of Obstetrics and Gynecology, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan.
Abstract
AIM: To investigate the relationship between the genotype of enzymes for detoxification and recurrent miscarriage. METHODS: The frequency of each genotype of enzymes for detoxification, such as cytochrome P450-1A1 (CYP1A1), glutathione-S-transferase-pi (GST-pi), GST-mu and GST-theta, was analyzed in patients with unexplained recurrent miscarriage (number of miscarriages ≥3) by polymerase-chain reaction-restriction fragment length polymorphism. A total of 103 patients were enrolled in the study, and 101 normal fertile women, who had never suffered a miscarriage, were also enrolled. The frequency of each genotype of the enzymes was compared between the patient population and the control group. The χ(2) -test and Fisher's exact test were used for statistical evaluation. RESULTS: No significant difference was observed concerning the distribution of polymorphic variants among CYP1A1, GST-pi, GST-mu and GST-theta. On the other hand, the frequency of individuals with the GST-mu deletion was significantly higher in cases of recurrent miscarriage compared with the control population among coffee drinkers (61% vs 41%, P = 0.025). CONCLUSION: There is a possibility that lower GST-mu enzyme activity may represent a risk factor for recurrent miscarriage, especially in the patient population of coffee consumers, as a result of impaired placental detoxification.
AIM: To investigate the relationship between the genotype of enzymes for detoxification and recurrent miscarriage. METHODS: The frequency of each genotype of enzymes for detoxification, such as cytochrome P450-1A1 (CYP1A1), glutathione-S-transferase-pi (GST-pi), GST-mu and GST-theta, was analyzed in patients with unexplained recurrent miscarriage (number of miscarriages ≥3) by polymerase-chain reaction-restriction fragment length polymorphism. A total of 103 patients were enrolled in the study, and 101 normal fertile women, who had never suffered a miscarriage, were also enrolled. The frequency of each genotype of the enzymes was compared between the patient population and the control group. The χ(2) -test and Fisher's exact test were used for statistical evaluation. RESULTS: No significant difference was observed concerning the distribution of polymorphic variants among CYP1A1, GST-pi, GST-mu and GST-theta. On the other hand, the frequency of individuals with the GST-mu deletion was significantly higher in cases of recurrent miscarriage compared with the control population among coffee drinkers (61% vs 41%, P = 0.025). CONCLUSION: There is a possibility that lower GST-mu enzyme activity may represent a risk factor for recurrent miscarriage, especially in the patient population of coffee consumers, as a result of impaired placental detoxification.