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Literature DB >> 2156228

The B-A transition in superhelical DNA.

Abstract

Relaxation of a DNA superhelical stress due to the B to A transition induced by trifluoroethanol has been studied by assessing the change of DNA orientation in a flow gradient. Using DNAs of different superhelical densities, a decrease in the winding angle during the B----A shift of DNA was found to be 1.5 degrees per base pair in solution. Accepting the winding angle for B-DNA in solution to be 34.1 degrees, that for A-DNA must have a value of 32.6 degrees which agrees with the X-ray data for A-DNA in the condensed state. The date obtained within the B-A transition interval make it possible to conclude that there is an increase in winding at each B/A junction, which is about 5 degrees per one junction.

Entities: Chemical

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Year: 1990 PMID： 2156228 PMCID： PMC330324 DOI： 10.1093/nar/18.4.759

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

15 in total

1. The nature of the B-A transition of DNA in solution.

Authors: G Malenkov; L Minchenkova; E Minyat; A Schyolkina; V Ivanov
Journal: FEBS Lett Date: 1975-03-01 Impact factor: 4.124

2. Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNAase I in the presence of ethidium bromide.

Authors: L Greenfield; L Simpson; D Kaplan
Journal: Biochim Biophys Acta Date: 1975-10-15

3. A STUDY OF CONFORMATION OF NUCLEIC ACIDS IN SOLUTION BY MEANS OF CIRCULAR DICHROISM.

Authors: J BRAHMS; W F MOMMAERTS
Journal: J Mol Biol Date: 1964-10 Impact factor: 5.469

4. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

Authors: J C Wang
Journal: J Mol Biol Date: 1974-11-15 Impact factor: 5.469

The B-A transition in superhelical DNA.

1. The nature of the B-A transition of DNA in solution.

2. Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNAase I in the presence of ethidium bromide.

3. A STUDY OF CONFORMATION OF NUCLEIC ACIDS IN SOLUTION BY MEANS OF CIRCULAR DICHROISM.

4. The degree of unwinding of the DNA helix by ethidium. I. Titration of twisted PM2 DNA molecules in alkaline cesium chloride density gradients.

5. Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism.

6. Helical repeat of DNA in solution.

7. Sequence dependence of the helical repeat of DNA in solution.

8. On the flexibility of the boundaries between the A-form and B-form sections in DNA molecule.

9. Dehydrated circular DNA: circular dichroism of molecules in ethanolic solutions.

10. Statistical mechanical analysis of competing conformational transitions in superhelical DNA.

1. The detection of B-form/A-form junction in a deoxyribonucleotide duplex.