Literature DB >> 21562101

Cytomegalovirus DNA quantification using an automated platform for nucleic acid extraction and real-time PCR assay setup.

Michael Forman1, Andy Wilson, Alexandra Valsamakis.   

Abstract

Analytical performance characteristics of the QIAsymphony RGQ system with artus cytomegalovirus (CMV) reagents were determined. Measurable range spanned 2.0 to ≥ 7.0 log(10) copies/ml. The detection limit was 23 copies/ml. Intrarun and interrun coefficients of variation were ≤ 2.1% at 3.0 and 5.0 log(10) copies/ml. In clinical specimens, RGQ values were ~0.2 log(10) copies/ml higher than those in an assay using a BioRobot M48 extraction/manual reaction setup/7500 Real-Time PCR instrument. No cross-contamination was observed.

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Year:  2011        PMID： 21562101      PMCID： PMC3147859          DOI： 10.1128/JCM.00721-11

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  4 in total

1.  Real-time automated PCR for early diagnosis and monitoring of cytomegalovirus infection after bone marrow transplantation.

Authors:  U Machida; M Kami; T Fukui; Y Kazuyama; M Kinoshita; Y Tanaka; Y Kanda; S Ogawa; H Honda; S Chiba; K Mitani; Y Muto; K Osumi; S Kimura; H Hirai
Journal:  J Clin Microbiol       Date:  2000-07       Impact factor: 5.948

Review 2.  Cytomegalovirus in hematopoietic stem cell transplant recipients.

Authors:  Per Ljungman; Morgan Hakki; Michael Boeckh
Journal:  Infect Dis Clin North Am       Date:  2010-06       Impact factor: 5.982

3.  Rapid quantitation of cytomegalovirus DNA in whole blood by a new molecular assay based on automated sample preparation and real-time PCR.

Authors:  Reinhard B Raggam; Michael Bozic; Helmut J F Salzer; Sandra Hammerschmidt; Cordula Homberg; Katharina Ruzicka; Harald H Kessler
Journal:  Med Microbiol Immunol       Date:  2010-06-18       Impact factor: 3.402

4.  International consensus guidelines on the management of cytomegalovirus in solid organ transplantation.

Authors:  Camille N Kotton; Deepali Kumar; Angela M Caliendo; Anders Asberg; Sunwen Chou; David R Snydman; Upton Allen; Atul Humar
Journal:  Transplantation       Date:  2010-04-15       Impact factor: 4.939
  4 in total
  7 in total

1.  Outcomes in Transplant Recipients Treated With Foscarnet for Ganciclovir-Resistant or Refractory Cytomegalovirus Infection.

Authors:  Robin K Avery; Ravit Arav-Boger; Kieren A Marr; Edward Kraus; Shmuel Shoham; Laura Lees; Brandon Trollinger; Pali Shah; Rich Ambinder; Dionysios Neofytos; Darin Ostrander; Michael Forman; Alexandra Valsamakis
Journal:  Transplantation       Date:  2016-10       Impact factor: 4.939

2.  Clinical significance of low cytomegalovirus DNA levels in human plasma.

Authors:  Jesse Waggoner; Dora Y Ho; Paolo Libiran; Benjamin A Pinsky
Journal:  J Clin Microbiol       Date:  2012-04-18       Impact factor: 5.948

3.  Outcomes of transplant recipients treated with cidofovir for resistant or refractory cytomegalovirus infection.

Authors:  Seema A Mehta Steinke; Mona Alfares; Alexandra Valsamakis; Shmuel Shoham; Ravit Arav-Boger; Laura Lees; Darin Ostrander; Michael S Forman; Audra Shedeck; Richard F Ambinder; Richard John Jones; Robin K Avery
Journal:  Transpl Infect Dis       Date:  2020-12-02

4.  Comparison of automated nucleic acid extraction methods for the detection of cytomegalovirus DNA in fluids and tissues.

Authors:  Jesse J Waggoner; Benjamin A Pinsky
Journal:  PeerJ       Date:  2014-04-08       Impact factor: 2.984

5.  Automated Nucleic Acid Extraction Systems for Detecting Cytomegalovirus and Epstein-Barr Virus Using Real-Time PCR: A Comparison Study Between the QIAsymphony RGQ and QIAcube Systems.

Authors:  Hanah Kim; Mina Hur; Ji Young Kim; Hee Won Moon; Yeo Min Yun; Hyun Chan Cho
Journal:  Ann Lab Med       Date:  2017-03       Impact factor: 3.464

6.  Cytomegalovirus in Adult Allogeneic Blood and Marrow Transplant Patients Before or Around the Period of Neutrophil Recovery: A Single-Center, Retrospective, Descriptive Study.

Authors:  Isabella Martin; Alexandra Valsamakis; Douglas Gladstone; Richard Jones; Richard Ambinder; Robin K Avery
Journal:  Open Forum Infect Dis       Date:  2020-03-09       Impact factor: 3.835

7.  Comparative evaluation of the QIAsymphony RGQ system with the easyMAG/R-gene combination for the quantitation of cytomegalovirus DNA load in whole blood.

Authors:  Sylvie Pillet; Thomas Bourlet; Bruno Pozzetto
Journal:  Virol J       Date:  2012-10-09       Impact factor: 4.099
  7 in total

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