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Literature DB >> 2156

Amino acids attached to transfer ribonucleic acid in vivo.

Abstract

1. tRNA was extracted from rabbit liver by both the phenol and diethyl pyrocarbonate methods under conditions preventing deacylation of the amino acids attached in vivo. 2. After deacylation 12 amino acids were determined by gas-liquid chromatography, by using the flame-ionization and nitrogen-sensitive thermionic detectors. 3. Comparison of the distribution of 12 amino acids attached to tRNA with those contained in total tissue protein and in the free pool showed little correlation. 4. Results for the enzymic charging assay for tRNA in vitro did not correlate satisfactorily with the analysis of amino acids attached to tRNA in vivo. Marked differences were ntoed in comparison made between our own and other published results.

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Year: 1975 PMID： 2156 PMCID： PMC1165757 DOI： 10.1042/bj1500419

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

57 in total

6. Regulatory significance of transfer RNA charging levels. I. Measurements of charging levels in livers of chow-fed rats, fasting rats, and rats fed balanced or imbalanced mixtures of amino acids.

Authors: R E Allen; P L Raines; D M Regen
Journal: Biochim Biophys Acta Date: 1969-10-22

Amino acids attached to transfer ribonucleic acid in vivo.

1. ON THE STABILITY OF AMINOACYL-S-RNA TO NUCLEOPHILIC CATALYSIS.

2. Thermal conversion of nonpriming deoxyribonucleic acid to primer.

3. BINDING OF AMINO ACIDS TO THE END GROUP OF A SOLUBLE RIBONUCLEIC ACID.

4. Diethyl pyrocarbonate, a new tool in the chemical modification of nucleic acids?

5. Quantitative differences in proline tRNA content of rat liver and granulation tissue.

6. Regulatory significance of transfer RNA charging levels. I. Measurements of charging levels in livers of chow-fed rats, fasting rats, and rats fed balanced or imbalanced mixtures of amino acids.

7. Determination of amino acids by gas-liquid chromatography with the nitrogen-sensitive thermionic detector.

8. Changes in seryl-tRNA formation in developing chick muscle.

9. Multiple isoaccepting transfer RNA's in a muouse plasma cell tumor.

10. The preparation of rat-liver soluble ribonucleic acid.

1. MDCK microcarrier cultured: seeding density effects and amino acid utilization.