M B C Kilander1, C Halleskog, G Schulte. 1. Section Receptor Biology & Signalling, Department of Physiology and Pharmacology, Karolinska Institutet, Stockholm, Sweden.
Abstract
AIM: The objective of this study was to compare the efficacy of different recombinant, commercially available Wingless/Int-1 (WNTs) with regard to WNT/β-catenin signalling, dishevelled (DVL) and G protein activation and the induction of cell proliferation in a microglia-like cell line called N13. METHODS: For detection of activated signalling molecules, cell lysates are analysed by immunoblotting. Furthermore, we used a [γ(35)S] GTP binding assay to monitor the exchange of GDP for GTP in heterotrimeric G proteins in N13 membrane preparations. Cell proliferation was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay measuring mitochondrial function, which is proportional to the amount of viable cells. RESULTS: Of the WNTs tested (WNT-3A, -4, -5A, -5B, -7A,-9B), only WNT-3A activated WNT/β-catenin signalling in N13 cells. All WNTs induced the formation of phosphorylated and shifted DVL (PS-DVL) and the activation of heterotrimeric G proteins with variable efficacies. WNT-5A and WNT-9B, which had the highest efficacy in the G protein assay, also induced N13 cell proliferation. CONCLUSION: WNTs show significant differences in their efficacy to activate β-catenin-dependent and -independent signalling. The WNTs tested are present during maturation of the central nervous system and/or in the adult brain and are thus potential regulators of microglia-mediated neuroinflammation.
AIM: The objective of this study was to compare the efficacy of different recombinant, commercially available Wingless/Int-1 (WNTs) with regard to WNT/β-catenin signalling, dishevelled (DVL) and G protein activation and the induction of cell proliferation in a microglia-like cell line called N13. METHODS: For detection of activated signalling molecules, cell lysates are analysed by immunoblotting. Furthermore, we used a [γ(35)S] GTP binding assay to monitor the exchange of GDP for GTP in heterotrimeric G proteins in N13 membrane preparations. Cell proliferation was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay measuring mitochondrial function, which is proportional to the amount of viable cells. RESULTS: Of the WNTs tested (WNT-3A, -4, -5A, -5B, -7A,-9B), only WNT-3A activated WNT/β-catenin signalling in N13 cells. All WNTs induced the formation of phosphorylated and shifted DVL (PS-DVL) and the activation of heterotrimeric G proteins with variable efficacies. WNT-5A and WNT-9B, which had the highest efficacy in the G protein assay, also induced N13 cell proliferation. CONCLUSION: WNTs show significant differences in their efficacy to activate β-catenin-dependent and -independent signalling. The WNTs tested are present during maturation of the central nervous system and/or in the adult brain and are thus potential regulators of microglia-mediated neuroinflammation.
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