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Literature DB >> 21553872

Rapid detection of a cocaine-binding aptamer using biological nanopores on a chip.

Ryuji Kawano¹, Toshihisa Osaki, Hirotaka Sasaki, Masahiro Takinoue, Satoko Yoshizawa, Shoji Takeuchi.

Abstract

This paper describes a methodology for the rapid and highly selective detection of cocaine using a membrane protein channel combined with a DNA aptamer. The DNA aptamer recognizes the cocaine molecule with high selectivity. We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip.

Entities: Chemical

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Year: 2011 PMID： 21553872 DOI： 10.1021/ja2026085

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

29 in total

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Journal: Nat Mater Date: 2012-11-18 Impact factor: 43.841

2. Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores.

Authors: Nicholas A W Bell; Ulrich F Keyser
Journal: Nat Nanotechnol Date: 2016-04-04 Impact factor: 39.213

3. Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.

Authors: Mark T Agasid; Troy J Comi; S Scott Saavedra; Craig A Aspinwall
Journal: Anal Chem Date: 2016-12-30 Impact factor: 6.986

4. A pumpless solution exchange system for nanopore sensors.

Authors: Tetsuya Yamada; Koki Kamiya; Toshihisa Osaki; Shoji Takeuchi
Journal: Biomicrofluidics Date: 2019-11-04 Impact factor: 2.800

Review 5. Membrane protein-based biosensors.

Authors: Nobuo Misawa; Toshihisa Osaki; Shoji Takeuchi
Journal: J R Soc Interface Date: 2018-04 Impact factor: 4.118

Rapid detection of a cocaine-binding aptamer using biological nanopores on a chip.

1. Self-assembled nanoparticle arrays for multiphase trace analyte detection.

2. Digitally encoded DNA nanostructures for multiplexed, single-molecule protein sensing with nanopores.

3. Enhanced Temporal Resolution with Ion Channel-Functionalized Sensors Using a Conductance-Based Measurement Protocol.

4. A pumpless solution exchange system for nanopore sensors.

Review 5. Membrane protein-based biosensors.

6. Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine).

7. Unzipping kinetics of duplex DNA containing oxidized lesions in an α-hemolysin nanopore.

8. Sampling a biomarker of the human immunodeficiency virus across a synthetic nanopore.

9. Solid-State and Biological Nanopore for Real-Time Sensing of Single Chemical and Sequencing of DNA.

10. Detection of two isomeric binding configurations in a protein-aptamer complex with a biological nanopore.