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Literature DB >> 21549325

Eyes wide open: a critical review of sphere-formation as an assay for stem cells.

Erika Pastrana¹, Violeta Silva-Vargas, Fiona Doetsch.

Abstract

Sphere-forming assays have been widely used to retrospectively identify stem cells based on their reported capacity to evaluate self-renewal and differentiation at the single-cell level in vitro. The discovery of markers that allow the prospective isolation of stem cells and their progeny from their in vivo niche allows the functional properties of purified populations to be defined. We provide a historical perspective of the evolution of the neurosphere assay and highlight limitations in the use of sphere-forming assays in the context of neurospheres. We discuss theoretical and technical considerations of experimental design and interpretation that surround the use of this assay with any tissue.

Entities: Chemical Disease Gene Species

Mesh：

Year: 2011 PMID： 21549325 PMCID： PMC3633588 DOI： 10.1016/j.stem.2011.04.007

Source DB: PubMed Journal: Cell Stem Cell ISSN： 1875-9777 Impact factor: 24.633

111 in total

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2. A novel classification of quiescent and transit amplifying adult neural stem cells by surface and metabolic markers permits a defined simultaneous isolation.

Authors: Franz-Josef Obermair; Roberto Fiorelli; Aileen Schroeter; Sarah Beyeler; Claudia Blatti; Bjoern Zoerner; Michaela Thallmair
Journal: Stem Cell Res Date: 2010-05-16 Impact factor: 2.020

3. Cellular composition and three-dimensional organization of the subventricular germinal zone in the adult mammalian brain.

Authors: F Doetsch; J M García-Verdugo; A Alvarez-Buylla
Journal: J Neurosci Date: 1997-07-01 Impact factor: 6.167

4. Network of tangential pathways for neuronal migration in adult mammalian brain.

Authors: F Doetsch; A Alvarez-Buylla
Journal: Proc Natl Acad Sci U S A Date: 1996-12-10 Impact factor: 11.205

5. Multipotent CNS stem cells are present in the adult mammalian spinal cord and ventricular neuroaxis.

Authors: S Weiss; C Dunne; J Hewson; C Wohl; M Wheatley; A C Peterson; B A Reynolds
Journal: J Neurosci Date: 1996-12-01 Impact factor: 6.167

6. Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.

Authors: Steven M Pollard; Koichi Yoshikawa; Ian D Clarke; Davide Danovi; Stefan Stricker; Roslin Russell; Jane Bayani; Renee Head; Marco Lee; Mark Bernstein; Jeremy A Squire; Austin Smith; Peter Dirks
Journal: Cell Stem Cell Date: 2009-06-05 Impact factor: 24.633

7. Rostro-caudal gradual loss of cellular diversity within the periventricular regions of the ventricular system.

Authors: Andreas Hermann; Christian Suess; Mareike Fauser; Sylvia Kanzler; Martin Witt; Klaus Fabel; Johannes Schwarz; Günter U Höglinger; Alexander Storch
Journal: Stem Cells Date: 2009-04 Impact factor: 6.277

Review 8. Regulation of self-renewal and differentiation in adult stem cell lineages: lessons from the Drosophila male germ line.

Authors: E L Davies; M T Fuller
Journal: Cold Spring Harb Symp Quant Biol Date: 2009-03-27

Review 9. The glial nature of embryonic and adult neural stem cells.

Authors: Arnold Kriegstein; Arturo Alvarez-Buylla
Journal: Annu Rev Neurosci Date: 2009 Impact factor: 12.449

10. CD133 (Prominin) negative human neural stem cells are clonogenic and tripotent.

Authors: Yirui Sun; Weiqing Kong; Anna Falk; Jin Hu; Liangfu Zhou; Steve Pollard; Austin Smith
Journal: PLoS One Date: 2009-05-11 Impact factor: 3.240

354 in total

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Journal: Genes Dev Date: 2012-06-01 Impact factor: 11.361

Review 2. Head and neck cancer stem cells.

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Journal: J Dent Res Date: 2011-09-20 Impact factor: 6.116

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Journal: Nat Protoc Date: 2011-11-03 Impact factor: 13.491

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10. Side population cells from long-term passage non-small cell lung cancer cells display loss of cancer stem cell-like properties and chemoradioresistance.

Authors: Hao Gu; Xin-Yu Wu; Rui-Tai Fan; Xin Wang; You-Zhong Guo; Rui Wang
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