OBJECTIVE: To study the effects and mechanism of Aconitum vaginatum on proliferation, invasion and metastasis of human lung adenocarcinoma A549 cell lines. METHODS: A549 cells were treated with Aconitum vaginatum at different concentrations, and divided into 4 groups: Aconitum vaginatum 0.01, 0.1, 1 mg/mL groups and control group, respectively. MTT assay was used to evaluate cell proliferation; migration and invasion (with matrigel) assay was conducted in the transwell chamber; gelatin zymography was performed to evaluated the impacts of Aconitum vaginatum on matrix metalloproteinase (MMP)-2, MMP-9. RESULTS: Aconitum vaginatum exhibited a concentration-dependent inhibitory on proliferation and invasion of A549 cells. Aconitum vaginatum was also found to decrease the activity of MMP-2, MMP-9 in a concentration-dependent manner (P < 0.05). CONCLUSION: Aconitum vaginatum can inhibit the proliferation, invasion and metastasis of human adenocarcinoma A549 cell lines, the mechanism maybe deceasing MMP-2, MMP-9 activity.
OBJECTIVE: To study the effects and mechanism of Aconitum vaginatum on proliferation, invasion and metastasis of humanlung adenocarcinoma A549 cell lines. METHODS: A549 cells were treated with Aconitum vaginatum at different concentrations, and divided into 4 groups: Aconitum vaginatum 0.01, 0.1, 1 mg/mL groups and control group, respectively. MTT assay was used to evaluate cell proliferation; migration and invasion (with matrigel) assay was conducted in the transwell chamber; gelatin zymography was performed to evaluated the impacts of Aconitum vaginatum on matrix metalloproteinase (MMP)-2, MMP-9. RESULTS: Aconitum vaginatum exhibited a concentration-dependent inhibitory on proliferation and invasion of A549 cells. Aconitum vaginatum was also found to decrease the activity of MMP-2, MMP-9 in a concentration-dependent manner (P < 0.05). CONCLUSION: Aconitum vaginatum can inhibit the proliferation, invasion and metastasis of humanadenocarcinoma A549 cell lines, the mechanism maybe deceasing MMP-2, MMP-9 activity.