Literature DB >> 21545786

Detection of glutathione in whole blood after stabilization with N-ethylmaleimide.

Daniela Giustarini1, Isabella Dalle-Donne, Aldo Milzani, Ranieri Rossi.   

Abstract

The measurement of glutathione is a challenging task in that, during sample manipulation, a large percentage of this compound can be artificially oxidized. Here a high-performance liquid chromatography (HPLC) method to measure reduced glutathione in blood, based on the analysis of its conjugate with N-ethylmaleimide, has been developed and validated. The method is linear in the concentration range 0.1-2 mM, and the lower limit of quantification is 0.05 mM. Blood samples treated with N-ethylmaleimide are stable at -20°C for 90 days. This method has simplicity and rapidity as its main advantages; therefore, it is particularly suitable for large-scale clinical studies.
Copyright © 2011 Elsevier Inc. All rights reserved.

Entities:  

Mesh：

Substances：

Year:  2011        PMID： 21545786     DOI： 10.1016/j.ab.2011.04.013

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  16 in total

1.  Micro-method for the determination of glutathione in human blood.

Authors:  Daniela Giustarini; Paolo Fanti; Elena Matteucci; Ranieri Rossi
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2014-02-19       Impact factor: 3.205

2.  Pitfalls in the analysis of the physiological antioxidant glutathione (GSH) and its disulfide (GSSG) in biological samples: An elephant in the room.

Authors:  Daniela Giustarini; Dimitrios Tsikas; Graziano Colombo; Aldo Milzani; Isabella Dalle-Donne; Paolo Fanti; Ranieri Rossi
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2016-02-12       Impact factor: 3.205

3.  Immediate stabilization of human blood for delayed quantification of endogenous thiols and disulfides.

Authors:  Daniela Giustarini; Federico Galvagni; Maurizio Orlandini; Paolo Fanti; Ranieri Rossi
Journal:  J Chromatogr B Analyt Technol Biomed Life Sci       Date:  2016-02-08       Impact factor: 3.205

4.  Extending the Scope of 1H NMR-Based Blood Metabolomics for the Analysis of Labile Antioxidants: Reduced and Oxidized Glutathione.

Authors:  G A Nagana Gowda; Vadim Pascua; Daniel Raftery
Journal:  Anal Chem       Date:  2021-10-27       Impact factor: 6.986

5.  Glutathione redox potential is low and glutathionylated and cysteinylated hemoglobin levels are elevated in maintenance hemodialysis patients.

Authors:  Khaled Khazim; Daniela Giustarini; Ranieri Rossi; Darlene Verkaik; John E Cornell; Sue E D Cunningham; Maryam Mohammad; Kara Trochta; Carlos Lorenzo; Franco Folli; Shweta Bansal; Paolo Fanti
Journal:  Transl Res       Date:  2013-01-17       Impact factor: 7.012

6.  Analysis of GSH and GSSG after derivatization with N-ethylmaleimide.

Authors:  Daniela Giustarini; Isabella Dalle-Donne; Aldo Milzani; Paolo Fanti; Ranieri Rossi
Journal:  Nat Protoc       Date:  2013-08-01       Impact factor: 13.491

7.  Reversible disulfide formation of the glutamate carboxypeptidase II inhibitor E2072 results in prolonged systemic exposures in vivo.

Authors:  Rana Rais; Randall Hoover; Krystyna Wozniak; Michelle A Rudek; Takashi Tsukamoto; Jesse Alt; Camilo Rojas; Barbara S Slusher
Journal:  Drug Metab Dispos       Date:  2012-09-04       Impact factor: 3.922

8.  Ratiometric Detection of Glutathione Based on Disulfide Linkage Rupture between a FRET Coumarin Donor and a Rhodamine Acceptor.

Authors:  Yibin Zhang; Shuai Xia; Shulin Wan; Tessa E Steenwinkel; Tara Vohs; Rudy L Luck; Thomas Werner; Haiying Liu
Journal:  Chembiochem       Date:  2021-05-13       Impact factor: 3.461

9.  Pharmacological targeting of glucose-6-phosphate dehydrogenase in human erythrocytes by Bay 11-7082, parthenolide and dimethyl fumarate.

Authors:  Mehrdad Ghashghaeinia; Daniela Giustarini; Pavla Koralkova; Martin Köberle; Kousi Alzoubi; Rosi Bissinger; Zohreh Hosseinzadeh; Peter Dreischer; Ingolf Bernhardt; Florian Lang; Mahmoud Toulany; Thomas Wieder; Renata Mojzikova; Ranieri Rossi; Ulrich Mrowietz
Journal:  Sci Rep       Date:  2016-06-29       Impact factor: 4.379

Review 10.  Glutathione as a Redox Biomarker in Mitochondrial Disease-Implications for Therapy.

Authors:  Gregory M Enns; Tina M Cowan
Journal:  J Clin Med       Date:  2017-05-03       Impact factor: 4.241
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.