| Literature DB >> 2154538 |
A R Smith1, M E Boursnell, M M Binns, T D Brown, S C Inglis.
Abstract
Nucleotide sequences from the third open reading frame of mRNA D (D3) of infectious bronchitis virus (IBV) were expressed in bacteria as part of a fusion protein with beta-galactosidase. Antiserum raised in rabbits against this fusion protein immunoprecipitated from IBV-infected chick kidney or Vero cells a polypeptide of 12.4K, the size expected for a D3-encoded product. The D3 polypeptide is apparently non-glycosylated, and appears to be associated with the membrane fraction of infected cells, as judged by cell fractionation and immunofluorescence.Entities:
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Year: 1990 PMID: 2154538 DOI: 10.1099/0022-1317-71-1-3
Source DB: PubMed Journal: J Gen Virol ISSN: 0022-1317 Impact factor: 3.891