AIM: HLA-DR monocyte expression may be affected by major surgery. A potential mechanism for monocyte activation is the engagement of costimulatory receptors (B7-2 or CD-86). The aim of the present study was to determine the possible role of monocyte HLA-DR and B7-2 molecules in the occurrence of postoperative sepsis after major cancer surgery. METHODS: This was an observational study in 25 consecutive patients undergoing major elective surgery. Flow cytometry measures were used to determine the expression of HLA-DR and its costimulatory receptors before (day 0) and after surgery (day 1 and day 2). RESULTS: After surgery, the rate of monocytes expressing HLA-DR decreased significantly in all the patients. As compared with day 0, the rate of monocytes expressing B7-2 decreased in all the patients (P<0.03). In the septic group, it remained significantly decreased postoperatively. In the non-septic group, it reached baseline levels at day 2. CONCLUSION: Results suggest a key role for costimulatory molecules in modulating inflammatory response in the context of subsequent postoperative sepsis after major cancer surgery. These molecules may be involved, in association with HLA-DR, in postoperative monocyte dysfunction.
AIM: HLA-DR monocyte expression may be affected by major surgery. A potential mechanism for monocyte activation is the engagement of costimulatory receptors (B7-2 or CD-86). The aim of the present study was to determine the possible role of monocyte HLA-DR and B7-2 molecules in the occurrence of postoperative sepsis after major cancer surgery. METHODS: This was an observational study in 25 consecutive patients undergoing major elective surgery. Flow cytometry measures were used to determine the expression of HLA-DR and its costimulatory receptors before (day 0) and after surgery (day 1 and day 2). RESULTS: After surgery, the rate of monocytes expressing HLA-DR decreased significantly in all the patients. As compared with day 0, the rate of monocytes expressing B7-2 decreased in all the patients (P<0.03). In the septic group, it remained significantly decreased postoperatively. In the non-septic group, it reached baseline levels at day 2. CONCLUSION: Results suggest a key role for costimulatory molecules in modulating inflammatory response in the context of subsequent postoperative sepsis after major cancer surgery. These molecules may be involved, in association with HLA-DR, in postoperative monocyte dysfunction.
Authors: Isaiah R Turnbull; Annie Hess; Anja Fuchs; Elfaridah P Frazier; Sarbani Ghosh; Shin-Wen Hughes; Michael P Kelly Journal: Spine Deform Date: 2022-06-14
Authors: J Shibata; S Ishihara; N Tada; K Kawai; N H Tsuno; H Yamaguchi; E Sunami; J Kitayama; T Watanabe Journal: Tech Coloproctol Date: 2015-03-12 Impact factor: 3.781
Authors: E Guillén-Gómez; L Guirado; X Belmonte; A Maderuelo; S Santín; C Juarez; E Ars; C Facundo; J A Ballarín; S Vidal; M M Díaz-Encarnación Journal: Clin Exp Immunol Date: 2014-02 Impact factor: 4.330
Authors: Gunnar Lachmann; Johannes Kurth; Clarissa von Haefen; Fatima Yuerek; Klaus-Dieter Wernecke; Claudia Spies Journal: Int J Med Sci Date: 2017-04-08 Impact factor: 3.738
Authors: Sebastian Weiterer; Florian Uhle; Sabin Bhuju; Michael Jarek; Markus A Weigand; Marek Bartkuhn Journal: PLoS One Date: 2014-04-14 Impact factor: 3.240
Authors: Gunnar Lachmann; Clarissa von Haefen; Johannes Kurth; Fatima Yuerek; Klaus-Dieter Wernecke; Claudia Spies Journal: Biomed Res Int Date: 2017-08-08 Impact factor: 3.411